A549 cells (CRM-CCL-185), which have some characteristic lung epithelial cells, and HFL-1 cells (CCL-153) were obtained from ATCC (Manassas, USA). BEAS-2B cells (GNHu27) were purchased from the Cell Bank of Type Culture Collection (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China). Cells were cultured in DMEM or RPMI 1640 containing 10% fetal bovine serum (Gibco/BRL, Grand Island, USA) and cultured in a 37°C humidified incubator under 5% CO
2. Negative control siRNA (NC), OGG1 siRNA, p53 siRNA, PCMV-C-FLAG vector (Vector-NC), OGG1 overexpression vector (PCMV-OGG1-FLAG) or OGG1 mutants were transfected into lung cells using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, USA) in accordance with the manufacturer’s standard protocol. The sequence information is shown in
Table 3 .
2. Negative control siRNA (NC), OGG1 siRNA, p53 siRNA, PCMV-C-FLAG vector (Vector-NC), OGG1 overexpression vector (PCMV-OGG1-FLAG) or OGG1 mutants were transfected into lung cells using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, USA) in accordance with the manufacturer’s standard protocol. The sequence information is shown in
siRNA | Sense (5′→3′) | Antisense (5′→3′) |
NC siRNA | UUCUCCGAACGUGUCACG | ACGUGACACGUUCGGAGAATT |
OGG1 siRNA | GCGCAAGUACUUCCAGCUATT | UAGCUGGAAGUACUUGCGCTT |
p53 siRNA-1 | CUACUUCCUGAAAACAACGTT | CGUUGUUUUUCAGGAAGUAGTT |
p53 siRNA-2 | GCGCACAGAGGAAGAGAAUTT | AUUCUCUUCCUCUGUGCGCCG |