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Sigma phaseprep bac dna kit

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The Sigma PhasePrep TM BAC DNA Kit is a laboratory equipment product designed for the extraction and purification of bacterial artificial chromosome (BAC) DNA. It provides a simple and efficient method to isolate BAC DNA from bacterial cultures.

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Lab products found in correlation

Lsm 880 laser scanning microscope, by Zeiss (2 mentions) Fluorescein 12 dutp, by Thermo Fisher Scientific (2 mentions) Prolong gold antifade, by Thermo Fisher Scientific (2 mentions) Lb broth plates, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PhasePrep BAC DNA Kit (13 citations) Sigma kits (5 citations)

2 protocols using sigma phaseprep bac dna kit

1

FISH Analysis of Mitotic Chromosomes

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Slides of mitotic chromosomes were prepared from imaginal discs of fourth instar larvae following published protocols3 (link),70 ,71 (link). BAC clones were obtained from the University of Liverpool19 or from a previously described BAC library72 (link). BACs were plated on agar plates (Thermo Fisher) and a single bacterial colony was used to grow an overnight bacterial culture in LB broth plates (Thermo Fisher) at 37 °C. DNA from the BACs was extracted using Sigma PhasePrep TM BAC DNA Kit (Sigma-Aldrich, NA-0100). BAC DNA for hybridization was labelled by nick translation with Cy3-, Cy5-dUTP (Enzo Life Sciences) or Fluorescein 12-dUTP (Thermo Fisher). Chromosomes were counterstained with DAPI in Prolong Gold Antifade (Thermo Fisher). Slides were analysed using a Zeiss LSM 880 Laser Scanning Microscope at 1,000× magnification. We note that localization of the M-locus to 1p11 is supported by both FISH and genomic analyses, but is contrary to a previously published placement at 1q2117 (link).
Matthews B.J., Dudchenko O., Kingan S.B., Koren S., Antoshechkin I., Crawford J.E., Glassford W.J., Herre M., Redmond S.N., Rose N.H., Weedall G.D., Wu Y., Batra S.S., Brito-Sierra C.A., Buckingham S.D., Campbell C.L., Chan S., Cox E., Evans B.R., Fansiri T., Filipović I., Fontaine A., Gloria-Soria A., Hall R., Joardar V.S., Jones A.K., Kay R.G., Kodali V.K., Lee J., Lycett G.J., Mitchell S.N., Muehling J., Murphy M.R., Omer A.D., Partridge F.A., Peluso P., Aiden A.P., Ramasamy V., Rašić G., Roy S., Saavedra-Rodriguez K., Sharan S., Sharma A., Smith M.L., Turner J., Weakley A.M., Zhao Z., Akbari O.S., Black WC I.V., Cao H., Darby A.C., Hill C.A., Johnston J.S., Murphy T.D., Raikhel A.S., Sattelle D.B., Sharakhov I.V., White B.J., Zhao L., Aiden E.L., Mann R.S., Lambrechts L., Powell J.R., Sharakhova M.V., Tu Z., Robertson H.M., McBride C.S., Hastie A.R., Korlach J., Neafsey D.E., Phillippy A.M, & Vosshall L.B. (2018). Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature, 563(7732), 501-507.
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2

Mitotic Chromosome Preparation and FISH Analyses

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Slides of mitotic chromosomes were prepared from imaginal discs of 4th instar larvae following published protocols3 (link),70 (link),71 (link). BAC clones were obtained from the University of Liverpool19 (link) or from a previously described BAC library72 (link). BACs were plated on agar plates (Thermo Fisher) and a single bacterial colony was used to grow an overnight bacterial culture in LB broth plates (Thermo Fisher) at 37oC. DNA from the BACs was extracted using Sigma PhasePrep TM BAC DNA Kit (Sigma-Aldrich, catalogue #NA-0100). BAC DNA for hybridization was labelled by nick translation with Cy3-, Cy5-dUTP (Enzo Life Sciences) or Fluorescein 12-dUTP (Thermo Fisher). Chromosomes were counterstained with DAPI in Prolong Gold Antifade (Thermo Fisher). Slides were analysed using a Zeiss LSM 880 Laser Scanning Microscope at 1000× magnification. We note that localization of the M-locus to 1p11 is supported by both FISH and genomic analyses, but is contrary to a previously published placement at 1q2117 (link).
Matthews B.J., Dudchenko O., Kingan S.B., Koren S., Antoshechkin I., Crawford J.E., Glassford W.J., Herre M., Redmond S.N., Rose N.H., Weedall G.D., Wu Y., Batra S.S., Brito-Sierra C.A., Buckingham S.D., Campbell C.L., Chan S., Cox E., Evans B.R., Fansiri T., Filipović I., Fontaine A., Gloria-Soria A., Hall R., Joardar V.S., Jones A.K., Kay R.G., Kodali V.K., Lee J., Lycett G.J., Mitchell S.N., Muehling J., Murphy M.R., Omer A.D., Partridge F.A., Peluso P., Aiden A.P., Ramasamy V., Rašić G., Roy S., Saavedra-Rodriguez K., Sharan S., Sharma A., Smith M.L., Turner J., Weakley A.M., Zhao Z., Akbari O.S., Black WC I.V., Cao H., Darby A.C., Hill C.A., Johnston J.S., Murphy T.D., Raikhel A.S., Sattelle D.B., Sharakhov I.V., White B.J., Zhao L., Aiden E.L., Mann R.S., Lambrechts L., Powell J.R., Sharakhova M.V., Tu Z., Robertson H.M., McBride C.S., Hastie A.R., Korlach J., Neafsey D.E., Phillippy A.M, & Vosshall L.B. (2018). Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature, 563(7732), 501-507.
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