Sebaceous glands were isolated from occiput hair follicles and transferred to Biocoat collagen type I-coated culture dishes (CORNING, Kennebunk, ME, USA). Informed written consent was acquired. The Medical Ethical Committee of the Kyungpook National University Hospital (Daegu, Korea) approved all of the described studies (IRB no. KNU 2018-0155). The explants were maintained in Dulbecco's Modified Eagle Medium (DMEM; Hyclone Laboratories, Logan, UT, USA) at 37℃ in a humidified atmosphere of 5% CO2. Explants were left for 4 days, and the medium was modified to Epilife (MEPI500CA; Gibco BRL, Grand Island, NY, USA). The cells were harvested with 0.25% trypsin/10-mM EDTA in Hank's Balanced Salt Solution (HBSS) and then sub-cultured.
ORS cells were isolated from hair shafts of hair follicles. Trimmed hair follicles were immersed in DMEM, which is supplemented with 20% fetal bovine serum in Biocoat collagen type I-coated culture dishes (CORNING). After culturing for 3 days, the medium was replaced with EpiLife. Cells from the second passage were utilized in the experiments.
ORS cells were isolated from hair shafts of hair follicles. Trimmed hair follicles were immersed in DMEM, which is supplemented with 20% fetal bovine serum in Biocoat collagen type I-coated culture dishes (CORNING). After culturing for 3 days, the medium was replaced with EpiLife. Cells from the second passage were utilized in the experiments.