Anti mouse il 6

Hesperetin was purchased from Cayman Chemicals (Ann Arbor, MI, USA) and β-CD and RAMEB were purchased from Wako Pure Chemical Industries (Osaka, Japan), respectively. Naringenin, DM-β-CD, dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), thiazolyl blue (MTT), compounds for phosphate buffer saline (PBS) and lipopolysaccharides (LPS) from Escherichia coli O111:B4 were obtained from Sigma-Aldrich (Darmstadt, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM) was obtained from Life Technologies (Carlsbad, CA, USA). Anti-Mouse IL-6 was purchased from eBioscience Inc. (San Diego, CA, USA). Human colon cancer (Caco-2) cells, breast cancer (MCF-7) cells and human cervical carcinoma (HeLa) cells were obtained from the American Type Cell Culture Collection (ATCC), USA.

Purified anti-human CD3 (OKT3) and purified anti-human CD28 (CD28.2) were purchased from eBioscience. All fluorochrome-conjugated antibodies (anti-human CD4 (RPA-T4), anti-human CD45RA (HI100), anti-human CD25 (BC96), anti-human FoxP3 (PCH101), anti-human IFNγ, anti-human IL-4, anti-human IL-17, and anti-mouse IL-6 were from eBioscience. Recombinant human IL-2 (202-IL), human TGFβ1 (240-B), and human latent TGFβ1 (299-LT) were purchased from R&D Systems. Anti-TGFβ (1D11.16.8), anti-CD25 (PC-61.5.3), and their isotype control antibodies (MOPC-21, HRPN) were from Bio X Cell. PGE2, TGFβ, and IL-6 ELISA Ready-SET-Go! kits were purchased from eBioscience.