Coulter epics elite esp

Fluorescence-activated cell sorting (FACS) analyses of sub-G₁ fractions using propidium iodide staining for DNA fragmentation. Briefly, MPT-S1 cells were harvested in 15 mL tubes, fixed with 70% ethanol, washed twice with PBS and stained with propidium iodide (PI) (50 μg/ml) and RNase A (100 μg/ml) for 30 min at 37 °C. At least 10,000 events were analyzed by flow cytometry (Coulter EPICS Elite ESP; Beckman Coulter, Fullerton, CA, USA) with the excitation set at 488 nm and emission at 610 nm. Data were analyzed using Flowing Software version 2.5.1 (Turku Bioscience, Turku, Finland).

Cells were grown in the presence of mAbs (10μg/mL) for 24 h and treated with the Coulter DNA-Prep reagents kit (Beckman Coulter). The red fluorescence was analyzed by flow cytometry with a Coulter Epics Elite ESP flow cytometer (Beckman Coulter). Cell cycle distribution was analyzed with the Multicycle-AV software (Phoenix Flow Systems).