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Caspase 1 p20 clone casper1

Manufactured by Adipogen
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Caspase-1 p20 (clone casper1) is a lab equipment product that can be used for the detection and quantification of caspase-1 p20 subunit. Caspase-1 is an enzyme involved in the inflammatory response and programmed cell death.

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Lab products found in correlation

β actin, by Merck Group (2 mentions) Gasdermin d, by Abcam (1 mentions) Il 1β af 401 na, by R&D Systems (1 mentions) Il 18, by Abcam (1 mentions) Irf 3 clone d83b9, by Cell Signaling Technology (1 mentions) Gapdh clone 14c10, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

2 protocols using caspase 1 p20 clone casper1

1

Immunoblotting for Inflammasome Proteins

Cited 2 times
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Immunoblotting was performed on cell lysates or precipitates from cell supernatants as described before (2 (link), 47 (link)) with the following the antibodies; caspase-1 p20 (clone casper1; Adipogen), IL-1β (AF-401-NA; R&D Systems), gasdermin D (EPR19828; Abcam), mouse caspase-11 (17D9; Cell Signaling), Stx2 A subunit (11E10; Thermo Scientific), human gasdermin D (EPR19829; Abcam), IL-18 (Biovision), and β-actin (Sigma-Aldrich).
Havira M.S., Ta A., Kumari P., Wang C., Russo A.J., Ruan J., Rathinam V.A, & Vanaja S.K. (2020). Shiga toxin suppresses noncanonical inflammasome responses to cytosolic LPS. Science immunology, 5(53), eabc0217.
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2

LPS-induced Inflammatory Signaling Pathway

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BMDM were seeded in 24-well plates and treated with E. coli or C. trachomatis LPS. Cells were lysed with RIPA buffer. Proteins in culture supernatants were precipitated with 7.2% trichloroacetic acid plus 0.15% sodium cholate and were resuspended with NuPAGE LDS sample buffer (12 (link)). Samples were subjected to SDS-PAGE and transferred to either PVDF or nitrocellulose for immunoblotting, respectively. Blots were blocked overnight at 4°C in PBS with 0.1% Tween 20 and 5% nonfat dry milk. Primary antibodies used include WN1 222-5 (Hycult Biotech), NF-κB (clone L8F6; Cell Signal), pNF-κB (clone 93H1; Cell Signal), IRF3 (clone D83B9; Cell Signal), pIRF3Ser396 (clone 4D4G; Cell Signal), GAPDH (clone 14C10; Cell Signal), pro-GSDMD (clone A-7; Santa Cruz), GSDMD (p30) (Abcam), caspase-1 (p20) (clone Casper-1; AdipoGen), pro-IL-18 (Biovison), IL-18 (Biovison), pro-caspase-11 (clone 17D9; Sigma), and beta-actin (Sigma). Horseradish peroxidase-conjugated secondary antibodies were used for detection (Invitrogen).
Yang C., Briones M., Chiou J., Lei L., Patton M.J., Ma L., McClarty G, & Caldwell H.D. (2019). Chlamydia trachomatis Lipopolysaccharide Evades the Canonical and Noncanonical Inflammatory Pathways To Subvert Innate Immunity. mBio, 10(2), e00595-19.
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