The inhibitors used in this study were purchased from Cell Signaling Technology. Rabbit polyclonal anti‐HMGA2 antibody (ab97276), rabbit polyclonal anti‐Histone H3 antibody (ab1791), rabbit polyclonal anti‐Histone H3 (acetyl K9) antibody (ab10812), rabbit polyclonal anti‐Lamin B1 antibody (ab65986), HRP‐conjugated goat anti‐rabbit IgG (ab6721) and goat anti‐mouse IgG (ab205719) were all purchased from Abcam. Rabbit monoclonal anti‐Akt antibody (#4691) and rabbit monoclonal anti‐Akt (phosphor Ser473) antibody were purchased from Cell Signaling Technology. Rabbit polyclonal anti‐p300 (phosphor Ser1834) antibody (PA5‐64531) was purchased from Thermo Fisher Scientific, and all antibodies were diluted in accordance with 1:1000. Besides, Recombinant Human EGF Protein (236‐EG) was purchased from R&D systems.
Rabbit polyclonal anti histone h3 antibody ab1791
Manufactured by Abcam
Sourced in United States
Rabbit polyclonal anti-Histone H3 antibody (ab1791) is a laboratory reagent used to detect and study Histone H3 protein in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human egf protein 236 eg, by R&D Systems (2 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions)
Huh 7, by National Collection of Authenticated Cell Cultures (1 mentions)
Snu 368, by National Collection of Authenticated Cell Cultures (1 mentions)
Snu 739, by National Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using rabbit polyclonal anti histone h3 antibody ab1791
1
Immunoblotting for HMGA2 and Akt Signaling
2
Inhibitor Evaluation for HMGA2 Signaling
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The inhibitors used in this study and the anti-HMGA2 antibody (#8179) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal anti-Histone H3 antibody (ab1791), rabbit polyclonal anti-Histone H3 (acetyl K9) antibody (ab10812), mouse monoclonal anti-β-actin antibody (ab8226), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (ab6721) and goat anti-mouse IgG (ab205719) were all purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal anti-p300 (phosphor S1834) antibody (PA5-64531) was purchased from Thermo Fisher Scienti c (Waltham, MA, USA). Besides, Recombinant Human EGF Protein (236-EG) was purchased from R&D systems (Minneapolis, MN, USA).
Cell lines and cell culture SNU-368, SNU-739, Huh-7 and HepG2 cells were obtained from the Cell Bank of the Chinese Academy of Science (Shanghai, China). Cells were cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% FBS (HyClone, Logan, UT, USA) at 37 °C in a humidi ed incubator containing 5% CO 2 .
Cell lines and cell culture SNU-368, SNU-739, Huh-7 and HepG2 cells were obtained from the Cell Bank of the Chinese Academy of Science (Shanghai, China). Cells were cultured in Dulbecco's modi ed Eagle's medium (DMEM) supplemented with 10% FBS (HyClone, Logan, UT, USA) at 37 °C in a humidi ed incubator containing 5% CO 2 .
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