Minispin plus microcentrifuge

Bulk 2H–MoS₂ powder (∼6 μm to max. 40 μm, product number 69860, batch number WXBD2352V) and single-walled carbon nanotubes (SWCNTs, 0.78 nm average diameter, product number 773735, batch number MKCJ7287) were purchased from Sigma Aldrich and used without further purification. Ultrapure type I water (18.2 MΩ cm) from a Millipore Simplicity® water purification system was used for all experiments. All organic solvents were HPLC grade and used without further purification. A bath sonicator (Elmasonic P60H ultrasonic cleaner) was used for sonication and an Eppendorf MiniSpin Plus Microcentrifuge was used for centrifugation.

The blood samples were collected from retro-orbital plexus using ether anaesthesia.⁴⁵ Blood collected into the Clot Activator tubes or Fluoride tubes (for glucose test) was stored at 4 °C for 3 h and then centrifuged at 3000 revolutions per minute for 10 min using MiniSpin Plus Microcentrifuge (Eppendorf, Germany) to separate the serum.⁴⁶ (link) The isolated serum samples were used to measure the levels of fasting blood glucose (FBG), SGPT, SGOT, total bilirubin, cholesterol, triglyceride, creatinine, uric acid, urea and lactate dehydrogenase (LDH). All the parameters were assessed routinely on the 0^th, 7th, 14th, 21st and 28th day using Standard Assay Erba Diagnostic Kits (Erba Semi-Auto Biochemistry Analyzer, UK). In addition, body weight of rats was also measured regularly using digital weighing balance.