The bacteria cells were cultured in 100 mL LB medium for 24 h in the presence of 20 mg/L of Pb (II) and collected by centrifuge at 8000 × g for 10 min, followed by suspension in saline buffer. Three different staining dyes were sequentially added to the cell suspension to stain the DNA (DAPI Nucleic Acid Stain, Molecular probes, Invitrogen), EPS (Wheat Germ Agglutinin, Alexa Fluor 633 Conjugate, Molecular Probes, Invitrogen), and Pb (II) (Leadmium Green AM Dye, Molecular Probes, Invitrogen). The samples were analyzed with a CLSM (Nikon A1 and Ti-E) equipped with a Plan Apo VC × 60 objective lens (NA 1.40, Nikon).
Plan apo vc 60 objective lens
Manufactured by Nikon
The Plan Apo VC × 60 objective lens is a high-performance microscope objective designed for advanced laboratory applications. It features a numerical aperture of 1.40 and a working distance of 0.13 mm, providing excellent optical performance and resolution. The lens is made with fluorite and low-dispersion glass elements to minimize chromatic aberrations and deliver sharp, clear images across a wide field of view.
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Lab products found in correlation
2 protocols using plan apo vc 60 objective lens
1
Multicolor Imaging of Pb-Stressed Bacteria
2
RNA FISH and Immunofluorescence Dual Labeling
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Cell samples plated on coverslips precoated with poly-l -lysine (SIGMA) were fixed in 4% PFA and then processed for RNA FISH and immunofluorescence as described previously (Naganuma et al. 2012 (link)). Double fluorescence TSA was carried out using FITC-labeled doRPS12 and DIG-labeled doSRSF1 RNA probes detected with HRP-labeled anti-fluorescein and Cy3-conjugated Tyramide or HRP-labeled anti-digoxigenin antibodies with Cy5-conjugated Tyramide (PerkinElmer). After probe hybridization and washing, the TSA procedure was performed in accordance with the manufacturer's protocol, adding a 30-min treatment with 1% of H2O2 between two TSAs to quench residual peroxidase activity from the first TSA reaction. Confocal images were acquired under a FLUOVIEW FV1000 confocal laser scanning microscope (Olympus) and a Nikon Confocal system A1Rsi (Nikon) equipped with a Plan Apo VC 60× objective lens (NA 1.40, Nikon).
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