Antibodies against the following proteins were used: polyclonal rabbit HSF1 (Cell Signaling, 4356), monoclonal rabbit pS326 HSF1 (Abcam, 76076), monoclonal mouse HSP90AB1 (GeneTex, 84342), monoclonal rabbit HSPA8 (Cell signaling, 8444), monoclonal rabbit NOTCH1 (Cell Signaling, 4147), monoclonal mouse Actin (Millipore, MAB1501) and polyclonal goat Lamin A (Santa Cruz biotechnology, sc-6214). The shRNAs used against HSF1 and HSF1 positively regulated targets are described in Supplementary Table 3 . To generate virus, we transfected HEK293T cells with a plasmid (pLKO.1) expressing the shRNA of interest and lentiviral packaging plasmids. Viral supernatant was collected over a period of 72 h and used for the transduction of T-ALL cells. For survival assays, the cells were infected twice and selected with puromycin starting 2 days after viral infection. For RNA-seq experiments and to avoid the effects of apoptosis due to HSF1 knockdown, infection conditions were optimized to reach ~90% transduction efficiency and RNA was collected 48 h after infection.
Monoclonal rabbit hspa8
Manufactured by Cell Signaling Technology
Monoclonal rabbit HSPA8 is an antibody product from Cell Signaling Technology. It recognizes the HSPA8 protein, which is a member of the heat shock protein family and is involved in various cellular processes such as protein folding and trafficking.
Automatically generated - may contain errors
Lab products found in correlation
Polyclonal rabbit hsf1, by Cell Signaling Technology (2 mentions)
Monoclonal rabbit ps326 hsf1, by Abcam (2 mentions)
Monoclonal mouse hsp90ab1, by GeneTex (2 mentions)
Monoclonal rabbit notch1, by Cell Signaling Technology (2 mentions)
Monoclonal mouse actin, by Merck Group (2 mentions)
Polyclonal goat lamin a, by Santa Cruz Biotechnology (2 mentions)
2 protocols using monoclonal rabbit hspa8
1
Evaluating HSF1 Regulatory Targets in T-ALL
2
Evaluating HSF1 Regulatory Targets in T-ALL
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Antibodies against the following proteins were used: polyclonal rabbit HSF1 (Cell Signaling, 4356), monoclonal rabbit pS326 HSF1 (Abcam, 76076), monoclonal mouse HSP90AB1 (GeneTex, 84342), monoclonal rabbit HSPA8 (Cell signaling, 8444), monoclonal rabbit NOTCH1 (Cell Signaling, 4147), monoclonal mouse Actin (Millipore, MAB1501) and polyclonal goat Lamin A (Santa Cruz biotechnology, sc-6214). The shRNAs used against HSF1 and HSF1 positively regulated targets are described in Supplementary Table 3 . To generate virus, we transfected HEK293T cells with a plasmid (pLKO.1) expressing the shRNA of interest and lentiviral packaging plasmids. Viral supernatant was collected over a period of 72 h and used for the transduction of T-ALL cells. For survival assays, the cells were infected twice and selected with puromycin starting 2 days after viral infection. For RNA-seq experiments and to avoid the effects of apoptosis due to HSF1 knockdown, infection conditions were optimized to reach ~90% transduction efficiency and RNA was collected 48 h after infection.
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