Multi reax vibrating shaker

For bacterial isolation, the protocols from Kucheryava et al. [45 (link)] and Barrientos-Díaz et al. [46 (link)] with modifications were used. Four grams of soil near to the root or 4 g of a mixture of leaves, stems, flowers, or fruits of each sampled plant were mixed with 40 mL of saline solution (NaCl 0.85% w v⁻¹) and stirred for 1 h in a Multi Reax vibrating shaker (Heidolph Instruments, Schwabach, Germany). Then, the suspensions were serially diluted (10⁻¹, 10⁻², 10⁻³, and 10⁻⁴) and 100 µL of each dilution were spread on TSA (pH 7) plates, supplemented with cycloheximide (100 µg mL⁻¹) to inhibit the growth of fungi. The plates were incubated at 4 °C for 2 weeks. Morphologically distinct individual colonies were individually seeded on fresh TSA plates at least twice to isolate pure strains. The colonies of pure cultures were grown in TSA medium at room temperature (22 ± 3 °C) for 24–48 h, and stored in 15% glycerol (v v⁻¹) at −20 and −80 °C.

Undecylenic acid (UDA), 2-(N-morpholino)ethanesulfonic acid (MES) hydrate, hydrofluoric acid (HF), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), tetrahydrofuran (THF), dimethyl sulfoxide (DMSO), chloroform, tert-butyloxycarbonyl-NH-PEG-Amine (BOC-NH-PEG-NH2), trifluoroacetic acid (TFA) were purchased from Sigma Aldrich (St. Louis, MO, USA). Graphene oxide was purchased from Biotool.com (Houston, TX, USA) as a batch of 2 mg mL⁻¹ in water with a nominal sheets size between 50 and 200 nm. Phosphoramidites for ONs syntheses were purchased from Glen Research (Sterling, VA, USA). PNA monomers were purchased from Link technologies (Bellshill, Lanarkshire, UK). Fmoc-L-Lys(MMT)-OH was purchased from Iris Biotech GmbH (Marktredwitz, Germany). All solvents for the syntheses of DNA and PNA molecules and MBHA resin (1% divinylbenzene, 200–400 mesh, 0.5 mmol/g loading) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). The solid-phase reactions were carried out using ISOLUTE^® single fritted reservoirs (SG), 3 mL 20 µm PE (polyethylene), provided with tube caps, and luer tip caps (Biotage, Uppsala, Sweden) which were shaken in a Multi-reax vibrating shaker (Heidolph, Schwabach, Germany).