Sulforaphane

Synthetic siRNA for MerTK and nonspecific control siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against lamin B1 and MerTK were obtained from Abcam (Cambridge, UK). Anti-NF-κB was obtained from Cell Signaling Technology (Danvers, MA, USA). Sandwich enzyme-linked immunosorbent assay (ELISA) kits for human TNF-α and IL-1β were purchased from BD Biosciences (San Diego, CA, USA). Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG were obtained from Jackson ImmunoResearch (West Grove, PA, USA). Sulforaphane and BAY 11-7082 were also acquired from Abcam. Pre-immune IgG was obtained from BioLegend (San Diego, CA, USA). Recombinant proteins of human TNF-α, IL-1β, and monocyte chemoattractant protein-1 (MCP-1) were purchased from R&D Systems (Minneapolis, MN, USA). Actinomycin D (inhibitor of de novo mRNA expression) and cycloheximide (inhibitor of protein synthesis) were obtained from Calbiochem (La Jolla, CA, USA). Ionomycin (Ca²⁺ ionophore) and thapsigargin (an endoplasmic reticulum Ca²⁺ pump inhibitor) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Anti-β-actin antibody and other chemicals were also acquired from Sigma-Aldrich.