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P perk

Manufactured by Proteintech
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Sourced in China

The P-PERK is a lab equipment product used to detect and measure the phosphorylation of the PERK protein, a key component of the unfolded protein response pathway. The product provides a reliable and efficient means to analyze this important cellular signaling event without making claims about its intended use.

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Lab products found in correlation

P erk, by Proteintech (2 mentions) Ire1α, by Abcam (1 mentions) P ire1α, by Abcam (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Grp94, by Proteintech (1 mentions) Grp78, by Proteintech (1 mentions) Caspase 12, by Proteintech (1 mentions) P erk, by Abcam (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Caspase 3, by Proteintech (1 mentions) Protein extraction kit, by Beyotime (1 mentions) Tsg101, by Proteintech (1 mentions) P eif2α, by Proteintech (1 mentions) Ire1α, by Proteintech (1 mentions) Collagen 1, by Proteintech (1 mentions) Grp78 bip, by Proteintech (1 mentions) Eif2α, by Proteintech (1 mentions) Bcl2 associated x (bax), by Proteintech (1 mentions)

3 protocols using p perk

1

Protein Extraction and Western Blot Analysis

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Total proteins were isolated using a protein extraction kit (Beyotime, Nantong, China) according to standard protocols. The antibodies used were: CD63 (1:1000), TSG101 (1:1000), Alix (1:1000), GRP78 (1:2000), GRP94(1:1000), CHOP (1:1000), caspase-3 (1:500), caspase-12 (1:1000) (Proteintech), p-PERK (1:1000), PERK (1:1000), ATF6 (1:1000), p-IRE1α (1:1000), IRE1α (1:1000) (Abcam), ERK (1:1000), p-ERK (1:1000), AKT(1:1000), p-AKT (1:1000), and GAPDH (1:1000) (Cell Signaling Technology). Horseradish peroxidase (HRP) -conjugated secondary antibodies (Santa Cruz Biotechnology) were used and protein bands were visualized and detected using an enhanced chemiluminescence system. GAPDH was used for normalization. The experiments were performed in triplicate.
Liao Z., Luo R., Li G., Song Y., Zhan S., Zhao K., Hua W., Zhang Y., Wu X, & Yang C. (2019). Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo. Theranostics, 9(14), 4084-4100.
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2

Hepatic Fibrosis Pathway Regulation

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CPT (purity ≥ 98% purity by HPLC, Cat No. HY-N0174), were purchased from Med Chem Express LLC (Shanghai, China) (Figure 1A). Primary antibodies for α-SMA, Collagen I, eIF2α, p-eIF2α, p-PERK, PERK, IRE1-α, BAX, BCL2, CHOP, and GRP78/BIP and all secondary antibodies were purchased from Proteintech (China). ATF4, and XBP1 were purchased from Sigma (United States). The primers used in quantitative reverse transcription polymerase chain reaction (qRT-PCR) were purchased from Qingke Co. Ltd. (China) (Table 1).
Hou X.X., Li Y.W., Song J.L., Zhang W., Liu R., Yuan H., Feng T.T., Jiang Z.Y., Li W.T, & Zhu C.L. (2023). Cryptotanshinone induces apoptosis of activated hepatic stellate cells via modulating endoplasmic reticulum stress. World Journal of Gastroenterology, 29(17), 2616-2627.
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3

Oridonin and Protein Regulation Pathways

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Oridonin (C20H28O6, CAS No. 28957–04-2, purity: 98%) was purchased from Yuanye Bio-Technology Co. (Shanghai, China). Oridonin was dissolved in dimethyl sulfoxide (DMSO, Sigma, D2650) and kept at 4 °C. In Fig. 1A, the chemical structure is depicted. LF3 (CAS: 664969–54-4) was purchased from Selleck (https://www.selleck.cn/). Z-VAD-FMK (Z-VAD, HY-16658B), Chloroquine (CQ, HY-17589A), and 4-Phenylbutyric acid (4-PBA, HY-A0281) were purchased from MCE (https://www.medchemexpress.cn/). Primary antibodies: ATF4 (Proteintech, 10,835–1-AP), CHOP (Proteintech, 15,204–1-AP), TCF4 (Proteintech, 22,337–1-AP), CFTR (Proteintech, 20,738–1-AP), FYN (Proteintech, 66,606–1-Ig), YOD1 (ABclonal, A13270), IRE1α (Cell Signaling Technology, Cat# 3294), p-IRE1α (Novus Biologicals, NB100-2323), PERK (Proteintech, 24,390–1-AP), p-PERK (ABclonal, AP0886), TP53 (Proteintech, 60,283–2-Ig), Wnt (Abcam, Ab15251), β-catenin (Abcam, Ab16051), β-actin (Proteintech, 66,009–1-Ig). Secondary antibodies: HRP enzyme-labeled goat anti-rabbit IgG (Servicebio, GB23303) and HRP enzyme-labeled goat anti-mouse IgG (Servicebio, GB23301).
Zhou F., Gao H., Shang L., Li J., Zhang M., Wang S., Li R., Ye L, & Yang S. (2023). Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer. Journal of Experimental & Clinical Cancer Research : CR, 42, 150.
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