After 48 h siRNA transfection or 48 h adenovirus transduction, HUVECs were harvested and seeded in 96-well plates, pre-coated with 50 μl matrigel (Corning Matrigel matrix, growth factor reduced) per well, at the density of 8,000 cells per well. After culturing in full EGM-2 medium for 8 h, images were acquired for each well (at central position) at × 5 objective (DM3000 inverted microscope, Leica). Images were analysed by WimTube (Wimasis Image Analysis).
Dm3000 inverted microscope
Manufactured by Leica
The DM3000 is an inverted microscope manufactured by Leica. It is designed for high-performance imaging and observation of samples. The DM3000 features a stable, vibration-resistant stand and provides optical excellence for a variety of applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dm3000 inverted microscope
1
Angiogenic Potential of HUVECs
2
Angiogenesis Assay for WARS2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 10
In Vitro Model of Angiogenesis
Methods
Tube Formation Assay
After 48 hours siRNA transfection or 48 hours adenovirus transduction or siRNA gene knockdown, HUVECs were harvested and seeded in 96-well plates, pre-coated with 50 μl matrigel (Corning Matrigel matrix, growth factor reduced) per well, at the density of 8,000 cells per well. After culturing in full EGM-2 medium for 8 hours, images were acquired for each well (at central position) at 5× objective (DM3000 inverted microscope, Leica). Images were analysed by WimTube (Wimasis Image Analysis).
Results
Modulation of WARS2 led to an increase and/or decrease in the formation of blood vessels (i.e., angiogenesis). The loss of WARS2 function led to very significant reduction in angiogenesis, as measured by the formation of tubes of endothelial cells; the total tube length; and the numbers of branching points. The data can be seen in
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!