Technetium labeled macroaggregated albumin 99mtc maa

HMPAO (Ceretec®) was purchased from GE Healthcare (Arlington Heights, IL), and technetium-labeled macroaggregated albumin (^99mTc-MAA, particle sizes 20 – 40 µm) was purchased from Cardinal Health (Wauwatosa, WI). Antibody to myeloperoxidase (MPO) (Abcam # Ab9535) was used with secondary antibody (Abcam #6829 HRP) to identify MPO positive cells. Diethyl maleate (DEM) and other reagent grade chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

HMPAO (Ceretec®) was purchased in kit form from GE Healthcare (Arlington Heights, IL), and technetium-labeled macroaggregated albumin (^99mTc-MAA, particle sizes 20 – 40 μm) was purchased from Cardinal Health (Wauwatosa, WI). Antibodies were purchased from Abcam for GSH synthetase (cat # 124811), Bcl-2 (cat # ab59348), myeloperoxidase (MPO) (cat # ab188211), and 3-nitrotyrosine (3-NT) (cat # ab52309). Antibodies for interleukin-1β (IL-1β) and peroxiredoxin-1 (Prdx-1) were purchased from RD systems (cat # MAB5011) and Cell Signaling (cat # 8499), respectively.