Em tp ultramicrotome

Frontal cortex samples were isolated from mice with stroke and fixed in 2% glutaraldehyde and 4% paraformaldehyde in 0.1 M sodium cacodylate (pH 7.4), treated with 10% gelatin solution in sodium cacodylate buffer, and incubated with 2% osmium tetroxide. Sections were cut using a Leica EM TP ultramicrotome (Leica Microsystems) at 40 nm and placed within grids stained with a 1:1 mix of 3% uranyl acetate and 50% acetone for 30–60 s. Grids were imaged with a JEM 1400 transmission electron microscope (JEOL) at ×1200 for low magnification and ×12,000 for high magnification (unless otherwise noted) using Gatan Microscopy Suite software (Gatan).

Liver samples were isolated from WT and UOX-KO mice and fixed in 2% glutaraldehyde and 4% paraformaldehyde in 0.1-M sodium cacodylate (pH=7.4), treated with 10% gelatin solution in sodium cacodylate buffer, and incubated with 2% osmium tetroxide. Sections measuring 40 nm were cut using a Leica EM TP ultramicrotome (Leica Microsystems) and placed within grids stained with a 1:1 mixture of 3% uranyl acetate and 50% acetone for 30–60 s. The grids were imaged with a JEM 1400 transmission electron microscope (JEOL) at ×1,200 for low magnification and ×12,000 for high magnification (unless otherwise noted) using Gatan Microscopy Suite software (Gatan). The cells were identified as described previously (30 (link)).