Paeonol

Aspirin was purchased from by Bayer HealthCare with the batch number of J20130078). Enzyme-linked Immunosorbent Assay (ELISA) Kits including Rat interleukin 1 (IL-1), Rat S-100B protein, Rat nuclear factorκB (NF-κB), Rat neuron specific enolase (NSE), Rat high sensitive C reactive protein (HS-CRP), Rat estradiol (E2), Rat tumor necrosis factor-α (TNF-α), and Rat D-dimmer (D2D), were purchased from Nanjing Senbeijia Biological Technology Co., Ltd., Trizol was purchased from Invitrogen. Anti-AKT, Anti-PI3K, and Anti-β-actin antibody was all purchased from Abcam. All durgs (purity assay by HPLC ≥ 98%, power) including compounds of Emodin, Ginsenoside Rg1, Ginsenoside Rb1, Notoginsenoside R1, Baicalein, Geniposide, β-asarone, Taurine, Paeoniflorin, Paeonol, Apigenin, Rhein, and Catalpol, were purchased from Chengdu Must Bio-Technology Co., Ltd.

Chengdu Must Bio-Technology Co., Ltd. provided the compositions of the ECC-BYF III, which are icariin (MUST-17051810), astragaloside IV (MUST-17022804), nobiletin (MUST-16070901), 20-S-ginsenoside Rh1 (MUST-17030717), and paeonol (MUST-16071405). Aminophylline tablets were purchased from Xinhua Pharmaceutical Co., Ltd. (Shandong, China). Both ECC-BYF III and aminophylline were fully dissolved in 0.5% CMC-Na (sodium carboxymethyl cellulose; Hengxing Chemical Reagent Manufacturing Co., Ltd.; 500 g/bottle) and prepared into suspension before gavage. Acupuncture needles were purchased from Suzhou Acupuncture Supplies Co., Ltd. (Huanqiu Brand), and its specification is 0.30 mm × 13 mm, 100 pieces per box. The electroacupuncture apparatus was purchased from Suzhou Medical Supplies Factory Co., Ltd.