Human cervical cancer cell line (HeLa) was obtained from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), trypsin, and phosphate-buffered solution (PBS, pH 7.4) were all purchased from Hyclone (San Angelo, TX, USA). Taxol and PTX were donated by Beijing ShuangLu Pharmaceutical Co, Ltd. Peptides and fluorescein isothiocyanate (FITC)-labeled peptides were synthesized by GL Biochem (Shanghai, People’s Republic of China). Chlorpromazine (CPZ) was purchased from Heowns Biochemical Technology (Tianjin, People’s Republic of China), nystatin (Nys) from Topscience (Shanghai, People’s Republic of China), 2-deoxy-d -glucose (2-D-G) and dimethyl sulfoxide (DMSO) from Aladdin (Shanghai, People’s Republic of China), sodium azide (NaN3) from Micxy Chemical Co (Cheng Du, People’s Republic of China), paraformaldehyde (PFA), 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and heparin from Solarbio (Beijing, People’s Republic of China). Mouse anti-β-tubulin primary antibody and FITC-labeled goat anti-mouse secondary antibody were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Female BALB/c mice were purchased from Beijing HFK Bioscience Co, Ltd (Beijing, People’s Republic of China).
Mouse anti β tubulin primary antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The mouse anti-β-tubulin primary antibody is a laboratory reagent used to detect and quantify the β-tubulin protein in biological samples. It is a monoclonal antibody that specifically recognizes the β-tubulin subunit of the microtubule cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Solarbio (1 mentions)
Fitc labeled goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Heparin, by Solarbio (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Solarbio (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Te2000 inverted microscope, by Nikon (1 mentions)
Lab tek chamber slide, by Thermo Fisher Scientific (1 mentions)
Oil immersion objective, by Nikon (1 mentions)
Normal goat serum (ngs), by Agilent Technologies (1 mentions)
2 protocols using mouse anti β tubulin primary antibody
1
Cytotoxicity Evaluation of Taxol Analogs
2
Cytoskeletal Visualization in A549 Cells
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A549 cells were seeded on Lab-Tek™ Chamber Slides (Thermo Fisher Scientific) and incubated for 24 h in the presence or absence 2b. Cells were then fixed for 30 min. with 2% paraformaldehyde at room temperature and washed with Dulbecco's phosphate-buffered saline (DPBS).
After permeabilization with 0.25% Triton X-100 for 5 min., A549 cells were washed in DPBS and incubated for 1 h in DPBS containing 1% Bovine Serum Albumine (BSA) and 10% normal goat serum (DAKO cytomation) for blocking.
Cells were then incubated overnight at 4 °C with the mouse anti-β tubulin primary antibody (Thermo Fisher Scientific).
Thereafter, the cells were washed with 0.2% Tween in DPBS, and incubated simultaneous for 2 h at room temperature with Alexa-Fluor 647 goat anti-mouse IgG secondary antibody and Hoechst 33258 (Thermo Fisher Scientific). Cells were then washed with DPBS and incubated for 30 min at room temperature with Alexa-Fluor 488 Phalloidin (Thermo Fisher Scientific) to perform F-actin labeling. Fluorescence images were collected by Nikon TE2000 inverted microscope with Nikon 1.4 NA DIC optics, 60X oil immersion objective (Nikon).
After permeabilization with 0.25% Triton X-100 for 5 min., A549 cells were washed in DPBS and incubated for 1 h in DPBS containing 1% Bovine Serum Albumine (BSA) and 10% normal goat serum (DAKO cytomation) for blocking.
Cells were then incubated overnight at 4 °C with the mouse anti-β tubulin primary antibody (Thermo Fisher Scientific).
Thereafter, the cells were washed with 0.2% Tween in DPBS, and incubated simultaneous for 2 h at room temperature with Alexa-Fluor 647 goat anti-mouse IgG secondary antibody and Hoechst 33258 (Thermo Fisher Scientific). Cells were then washed with DPBS and incubated for 30 min at room temperature with Alexa-Fluor 488 Phalloidin (Thermo Fisher Scientific) to perform F-actin labeling. Fluorescence images were collected by Nikon TE2000 inverted microscope with Nikon 1.4 NA DIC optics, 60X oil immersion objective (Nikon).
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