Mesoporous silica (MS) with an average diameter of 71 ± 2.1 nm and pore diameter of 13.3 nm, triphenylphosphonium (TPP-COOH) and succinimidyl propionic acid PEG (NHS-mPEG) were purchased from Xi'an Ruixi Company. N-Hydroxysuccinimide (NHS), hematoporphyrin monomethyl ether (HMME), N-(3-dimethylamino-propyl)-N-ethylcarbodiimide hydrochloride (EDC) and CuCl2 were obtained from J&K Scientific. The Cell Counting Kit (CCK-8) and LIVE/DEAD Viability/Cytotoxicity Kit were obtained from Dojindo China Co., Ltd. 3-Aminopropyltrimethoxysilane (APTES) and 2,7-dichlorodihydrofluoresceindiacetate (DCFH-DA) were purchased from Sigma-Aldrich. Deionized water with a resistivity of 18.2 MΩ cm was obtained from a Milli-Q Gradient System (Millipore, Bedford, MA, U.S.).
3 aminopropyltrimethoxysilane aptes
Manufactured by Merck Group
Sourced in China
3-Aminopropyltrimethoxysilane (APTES) is a silane coupling agent that is commonly used in laboratory and research applications. It contains three methoxy groups and one amino group, which allow it to form covalent bonds with various substrates, including glass, metal, and polymeric surfaces. APTES is widely utilized for surface modification, functionalization, and adhesion promotion purposes in diverse fields such as materials science, biotechnology, and nanotechnology.
Automatically generated - may contain errors
Lab products found in correlation
Milli q gradient system, by Merck Group (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Cucl2, by J&K Scientific (1 mentions)
Ascorbic acid, by Yuanye Bio-Technology (1 mentions)
Acetonitrile, by Beijing Chemical Works (1 mentions)
Citric acid, by Beijing Chemical Works (1 mentions)
H2so4, by Beijing Chemical Works (1 mentions)
Tetraethoxysilane teos, by Merck Group (1 mentions)
N hydroxysuccinimide, by Yuanye Bio-Technology (1 mentions)
Rat tnf α elisa kit, by Elabscience (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Cadmium perchlorate hydrate, by Merck Group (1 mentions)
Cetyltrimethylammonium bromide ctab, by Merck Group (1 mentions)
Manganese 2 chloride, by Merck Group (1 mentions)
Nickel 2 chloride, by Merck Group (1 mentions)
Mercury 2 chloride, by Merck Group (1 mentions)
Copper 2 chloride, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Lead 2 chloride, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
5 protocols using 3 aminopropyltrimethoxysilane aptes
1
Mesoporous Silica-based Nanocarrier Design
2
Synthesis of Multifunctional Nanomaterials
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MTX, ascorbic acid, FA, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide (EDC) and HAuCl4 were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Tetraethoxysilane (TEOS) and (3-aminopropyl) trimethoxysilane (APTES) were purchased from Sigma-Aldrich (St. Louis, MO, USA). H2SO4, N, N-dimethylformamide, citric acid and acetonitrile (DMF) were obtained from Beijing Chemical Works (Beijing, China). 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Beyotime Institute of Biotechnology (Haimen, China). Fetal bovine serum (FBS) and RAW 264.7 cells were obtained from Wuhan Procell Biological Technology Co., Ltd (Wuhan, China). NaBH4, AgNO3, CTAB and complete Freund's adjuvant (CFA) were obtained from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China). Rat TNF-α ELISA kits and IL-6 ELISA kits were obtained from Elabscience Biotechnology Co., Ltd (Wuhan, China).
3
Synthesis of Metal Nanoparticles
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Tetrachloroauric acid, sodium borohydride, ascorbic acid, silver nitrate, cetyltrimethyl-ammoniumbromide (CTAB), mercury(II) chloride, cadmium perchlorate hydrate, lead(II) chloride, nickel(II) chloride, manganese(II) chloride and copper(II) chloride and (3-aminopropyl)trimethoxysilane (APTES) were obtained from Sigma-Aldrich (Ireland). All glassware was cleaned with piranha solution prior to nanorod synthesis. Milli-Q water (resistivity > 18 MΩ cm−1) was used for all the experiments.
4
Multifunctional Nanodiamonds for Cancer Therapy
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All chemicals and reagents were used without further purification unless otherwise noted. The nanodiamonds were purchased from Sino Crytal Micro Diamond Co. Ltd. (Zhengzhou, China). Ferric chloride hexahydrate (FeCl3·6H2O), morpholine ethyl sulfonic acid (MES), ethylene glycol (CH2OHCH2OH), polyethylene glycol, (3-aminopropyl)-trimethoxy silane (APTES), and sodium acetate (CH3COONa) were purchased from Sigma-Aldrich (Shanghai, China) and used as received. Doxorubicin hydrochloride (DOX·HCl) was supplied by Shanghai Jinhe Bio-Technology Co. Ltd (Shanghai, China). Dulbecco’s minimum essential medium (DMEM) cell culture medium, penicillin/streptomycin (PS), and fetal bovine serum (FBS) were bought from Gibco Invitrogen (New York, USA). Human lung adenocarcinoma A549 cells were purchased from the Chinese Academy of Sciences Cell Bank. Cell Counting Kit-8 (CCK-8 assay), 4′,6-diamidino-2-phenylindole (DAPI), Calcein-AM and propidium iodide (PI) were purchased from Summus (Zhengzhou, China). Deionized (DI) water was freshly obtained using the Milli-Q water purification system (Millipore, Bedford, MA, USA).
5
Preparation and Characterization of Siloxane-Modified Surfaces
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Electrolyte solutions were made by dissolving sodium chloride (NaCl, 99.5%, Sigma-Aldrich) or calcium chloride (CaCl2, 96%, anhydrous, Sigma-Aldrich) in ion-exchanged water from a Milli-ROPls unit connected to a Milli-Q plus 185 system and filtered through a 0.2 m Millipak filter at 25 °C. The resistivity of the purified water was 18.2 M cm, and the organic content was less than 3 ppb. Trimethoxy(octyl)silane (96%), (3-aminopropyl)trimethoxysilane (APTES, 99%), and (3-mercaptopropyl)trimethoxysilane (95%) were purchased from Sigma-Aldrich, Denmark. Thermally oxidized silicon wafers with a 100 nm thick SiO2 layer were purchased from WaferNet, Inc. USA. Silica particles with a diameter of approximately 15 μm, used for the colloidal probe measurements, were purchased from Kisker Biotech, Germany. Rectangular silicon cantilevers (NSC15/Al BS, MikroMasch, Estonia) were used for atomic force microscope (AFM) image measurements. Rectangular tipless cantilevers (CSC37/TIPLESS/Cr-Au, MikroMasch, Estonia) with the approximate dimensions of 250 m in length, 32.5 m in width, and normal spring constants approximately 0.09 N/m were used for AFM force measurements. The spring constants of the cantilevers were determined by the Sader method 26, 27 before attaching the silica particles on the cantilevers.
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