The MON1 L. infantum laboratory strain LV50 originating from a visceral leishmaniasis case (Laboratory of Molecular Epidemiology and Experimental Pathology, Institut Pasteur de Tunis) was used to test the effect of the chemical compounds. Promastigote cultures were inoculated in RPMI-1640 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, 100 U/mL streptomycin and 10% (v/v) heat-inactivated fetal bovine serum at 22 °C. Promastigotes were collected at the beginning of the stationary phase (day 5), counted, centrifuged and seeded at 106/mL in 10 mL of complete media [7 (link)].
The human myelomonocytic cell line, THP-1, was ordered from the American Type Culture Collection (ATCC, TIB-202). Cells were maintained in RPMI 1640/Glutamax-I media (Gibco BRL, Bleiswijk, The Netherlands) supplemented with 10% heat-inactivated fetal calf serum (Gibco, Bleiswijk, The Netherlands) plus penicillin G (100 U/mL) and streptomycin (100 g/mL). THP-1 cells were differentiated to macrophages after their treatment with 20 ng/mL phorbol 12-myristate 7-acetate (PMA) (Sigma, St. Louis, MO, USA) for 48 h at 37 °C, 5% CO2. The viability of THP-1 mature macrophage-like cells was determined to be >97% by the Trypan blue dye exclusion assay.
The human myelomonocytic cell line, THP-1, was ordered from the American Type Culture Collection (ATCC, TIB-202). Cells were maintained in RPMI 1640/Glutamax-I media (Gibco BRL, Bleiswijk, The Netherlands) supplemented with 10% heat-inactivated fetal calf serum (Gibco, Bleiswijk, The Netherlands) plus penicillin G (100 U/mL) and streptomycin (100 g/mL). THP-1 cells were differentiated to macrophages after their treatment with 20 ng/mL phorbol 12-myristate 7-acetate (PMA) (Sigma, St. Louis, MO, USA) for 48 h at 37 °C, 5% CO2. The viability of THP-1 mature macrophage-like cells was determined to be >97% by the Trypan blue dye exclusion assay.