Rabbit anti neuropilin 2

Primary antibodies used were as follows: mouse anti-Islet 1/2 (DSHB, clone 39.4D5, 1:500), mouse anti-HB9 (DSHB, clone 81.5C10, 1:500), mouse anti-neurofilament-M (DSHB, clone 2H3, 1:500), rabbit anti-neurofilament (Sigma, 1:500), rabbit anti-Krox20 (Covance, 1:200), rabbit anti-CGRP (Millipore, 1:250), rabbit anti-parvalbumin (Chemicon, 1:500), rabbit anti-GFAP (Sigma, 1:500), rabbit anti-neuropilin-2 (Cell Signaling, 1:250), chicken anti-βGal (Aves, 1:1000). Secondary antibodies were Alexa-fluor conjugates (AF488, AF594, and AF647, Life Technologies, 1:500). Isolectin-B₄ was used at 1:200 (Life Technologies).

The following primary antibodies were used for immunoblotting or immunofluorescence staining: goat anti-Lyve1 (R&D Systems, #AF2125; dilution 1:250), rabbit anti-phospho AKT (Ser 473, Cell Signaling, 4060S; dilution 1:1000), rabbit anti-AKT (Cell Signaling, 4685; dilution 1:1000), rabbit anti-phospho ERK1/2 (Cell Signaling, 4370; dilution 1:1000), rabbit anti-ERK1/2 (Cell Signaling, 4695; dilution 1:1000), rabbit anti-tubulin (Cell Signaling, 2148; dilution 1:2000), rabbit anti-GAPDH (Cell Signaling, 2118; dilution 1:1000), chicken anti-GFP (abcam ab13970; 1:4000), rat anti-endomucin (Santa Cruz, sc-65495; dilution 1:50), rabbit anti-Prox1 (AngioBio, 11-002; dilution 1:1000), rabbit anti-neuropilin-2 (Cell Signaling, 3366; dilution 1:500), rabbit anti-Runx1 (Abcam, ab92336; dilution 1:1000), and rabbit anti-KRAS^G12D (Cell Signaling, #14429; dilution 1:1000).