The pTA131 plasmid was used to generate chromosomal deletions38 (link). DNA Phusion Taq polymerase, restriction enzymes, and DNA ligase were purchased from New England BioLabs. Plasmids were initially transformed into E. coli DH5α cells; plasmids were then transformed into E. coli Dam– strain DL739 prior to Hfx. volcanii transformation. Plasmid preparations for both E. coli strains were performed using the PureLinkTM Quick Plasmid Miniprep Kit (Invitrogen). Hfx. volcanii transformations were performed using the polyethylene glycol (PEG) method55 . All oligonucleotides used to construct the recombinant plasmids are listed in Supplementary Table 1 .
Dna phusion taq polymerase
Manufactured by New England Biolabs
DNA Phusion Taq polymerase is a high-fidelity DNA polymerase designed for accurate DNA amplification. It exhibits both 5' to 3' DNA polymerase and 3' to 5' exonuclease proofreading activities, providing efficient and precise DNA synthesis.
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Lab products found in correlation
2 protocols using dna phusion taq polymerase
1
Constructing Chromosomal Deletions in Hfx. volcanii
2
Plasmid-mediated genetic engineering in Hfx. volcanii
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The pTA131 plasmid was used to generate chromosomal deletions47 (link) and the pTA963 plasmid was used for complementation and overexpression experiments33 (link). DNA Phusion-Taq polymerase, restriction enzymes, and DNA ligase were purchased from New England BioLabs. Plasmids were initially transformed into E. coli DH5α cells; plasmids were then transformed into E. coli DAM− strain DL739 prior to Hfx. volcanii transformation. Plasmid preparations for both E. coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen). Hfx. volcanii transformations were performed using the polyethylene glycol (PEG) method38 . All oligonucleotides used to construct the recombinant plasmids are listed in Table S4 .
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