Twelve rats were randomly divided into two groups: a sham operation (SHAM) group and a DLBP group. Rats in the DLBP group were subjected to a surgical procedure [23 (link)] that could induce unbalanced dynamic and static forces. The paraspinal musculature and ligaments, along with part of the facet joints, were excised to destroy the static and dynamic lumbar stabilizers. For rats in the SHAM group, a posterior skin incision was made. According to previous studies [23 (link),24 (link)], the lumbar IDD induced by unbalanced dynamic and static forces was significant in the rat from 6 weeks after surgery. Thus the assessment of the pain threshold was done 1 day before surgery (baseline) and at 4, 6, 8, 10 and 12 weeks after surgery. Subsequently, the rats were examined by X-ray radiography (KXO-32R, Toshiba, Japan) and 3.0-T MRI (Siemens, Erlangen, Germany) to assess the IDD. After the radiological examination, all animals were euthanized by an overdose pentobarbital injection. The L5/6 IVDs and the L3–L5 spinal cord segments were harvested for Western blot, immunohistochemical and immunofluorescent analysis.
Kxo 32r
Manufactured by Toshiba
Sourced in Japan
The KXO-32R is a quartz crystal oscillator manufactured by Toshiba. It is designed to generate a stable reference frequency for electronic circuits and systems. The device features a compact surface-mount package and operates on a power supply voltage.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using kxo 32r
1
Inducing Intervertebral Disc Degeneration in Rats
2
Radiographic Assessment of Bone Age
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Images were acquired using a Toshiba KXO-32R general radiography machine. Radiographs of the left hand were acquired using the following exposure settings: (1) 46–50 kVp, (2) 2.0 mAs, (3) SID 100 cm and (4) with no grid. Images were read on a vendor approved console. Independent BA estimations by two radiologists with 4 years of clinical experience in pediatric radiology. BA estimates were obtained using the Greulich and Pyle's Radiographic Atlas of Skeletal Development of the Hand and Wrist (1 ). Both radiologists were blinded to the CA of the patients.
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