Accu check compact glucometer

Part of male offspring at the age of 8 weeks from each group were randomly selected, and, then weighted and sacrificed after fasting overnight. The rest mice were killed at the age of 10 months. After testing fasting blood glucose level with blood obtained from the tail vein using an Accu-check compact glucometer (Roche Diagnostic GmbH, Mannheim, Baden-Württemberg, Germany), we collected the blood from mouse eyes. Plasma was prepared by centrifugation (2000 rpm, 10 minutes) and kept at −20 °C until use. Livers and the perigonadal white adipose tissue (pWAT) were removed, weighed, dissected, snap-frozen in liquid nitrogen, and kept at −80 °C until use.

To induce T1D, 10-week-old female NOD/ShiLtJ mice were fasted for 24 h and intraperitoneally injected with 180 mg/kg STZ (Sigma-Aldrich, St. Louis, MO) in 0.1 M Na-citrate buffer (pH 4.5), or with only 0.1 M citrate buffer. The blood glucose levels of the mice were determined using an Accu-Check™ Compact Glucometer on days 0, 3, 7, and 11 after STZ injection (Roche Diagnostics, Indianapolis, IN).