Fmoc asp otbu oh

PL-Wang resin (0.4 mmole/g) was obtained from Varian, Inc. (Shropshire, UK). Fmoc-Ser(tBu)-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Ala-OH, Fmoc-Lys(Boc)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Asn(trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Phe-OH, Fmoc-Gln(trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Arg(pbf)-OH, N,N′-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP) and triisopropylsilane (TIS) were obtained from GL Biochem Ltd. (Shanghai, China). Fmoc-Val-OH, Fmoc-Gly-OH, Fmoc-His(trt)-OH and N,N,N′,N′-tetramethyl-uronium-hexafluoro-phosphate (HBTU) were obtained from CS Bio Co. (Menlo Park, USA). Dimethylformamide (DMF), dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were obtained from Daejung Chemicals & Metals Co., Ltd. (Siheung, Korea). Anisole, piperidine and N,N-diisopropylethylamine (DIEA) were obtained from Sigma-Aldrich (St. Louis, USA). Trifluoroacetic acid (TFA) was obtained from Yakuri Pure Chemicals Co., Ltd. (Kyoto, Japan). Ether, Anhydrous was obtained from J.T. Baker (Center Valley, USA). Zoletil® (Virbac) and Rompun® (Bayer Parma) were obtained from SMP animal medicine (Suwon, Korea). Protease inhibitor cocktail was obtained from Roche Diagnostics (IN, USA). EDTA treated BD vacutainer® was obtained from Becton, Dickinson and Company (NJ, USA). Human Aβ42 Ultrasensitive ELISA kit was obtained from Invitrogen (CA, USA).

N-Fluorenyl-9-methoxycarbonyl (Fmoc) protected amino acids (Fmoc-Trp(Boc)-OH, Fmoc-Phe-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Asp(OtBu)-OH), Fmoc-Gly-OH, 2-chlorotrityl chloride resin (100–200 mesh, loading: 0.97 mmol g⁻¹, 1% DVB), N-hydroxybenzotriazole (HOBt) and o-benzotriazole-N,N,N′,N′-tetramethyluroniumhexafluorophosphate (HBTU) were purchased from GL Biochem. Ltd (Shanghai, China) and used as received. Trifluoroacetic acid (TFA) and diisopropylethylamine (DIEA) were provided by Aladdin Reagent Co. Ltd (Shanghai, China). Piperidine, N,N-dimethylformamide (DMF) and triisopropylsilane (TIS) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) and used as received. Rhodamine B was obtained from Aladdin. Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS), and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetra-zoliumbromide (MTT) were provided by Invitrogen Corp. All other chemical agents were purchased from Sinopharm Chemical Reagent Co., Ltd and used without any pre-treatment.

Fmoc-Asp(OtBu)-OH, 2-chlorotrityl chloride resin, and Fmoc-Cys(OtBu)-OH were purchased from GL Biochem Co, Ltd (Shanghai, China); 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), N,N-dimethylformamide (DMF), ethyldiisopropylamine (DIEA), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), glutathione (GSH), Verapamil, diethyl ether, 1-hydroxybenzotriazole (HOBt), and triisopropylsilane were purchased from Sinopharm Chemical Reagent Co, Ltd (Shanghai, China); Ellman's reagent, propidium iodide and 4',6-diamidino-2-phenylindole (DAPI) were purchased from J&K Scientific (Beijing, China); annexin V-FITC was purchased from BioVision Inc (Mountain View, CA, USA). TAT-PEG₅₀₀₀-SH (TAT sequence YGRKKRRQRRR) was synthesized by Qufu Liyang Biochem Co, Ltd (Qufu, China).