Tissues frozen in liquid nitrogen were collected, and RNA was extracted with on miRNeasy FFPE kit (Qiagen). MiRcute enhanced miRNA cDNA rst strand synthesis Kit (TIANGEN) was used to reverse transcribe the RNA into complementary DNA. The qRT-PCR analysis was performed using the qPCR miRcute enhanced miRNA uorescence quanti cation kit (TIANGEN). The results were normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. We conducted three replicate experiments. The primers were synthesized by Thermo sher (Table S1).
Mircute enhanced mirna cdna rst strand synthesis kit
Manufactured by Tiangen Biotech
The MiRcute enhanced miRNA cDNA first strand synthesis Kit is a laboratory product designed for the reverse transcription of mature microRNA (miRNA) molecules. It facilitates the conversion of miRNA into complementary DNA (cDNA) for subsequent amplification and analysis.
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Lab products found in correlation
2 protocols using mircute enhanced mirna cdna rst strand synthesis kit
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Quantitative miRNA Expression Analysis
2
Quantitative miRNA Expression Analysis
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Tissues frozen in liquid nitrogen were collected, and RNA was extracted with on miRNeasy FFPE kit (Qiagen). MiRcute enhanced miRNA cDNA rst strand synthesis Kit (TIANGEN) was used to reverse transcribe the RNA into complementary DNA. The qRT-PCR analysis was performed using the qPCR miRcute enhanced miRNA uorescence quanti cation kit (TIANGEN). The results were normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. We conducted three replicate experiments. The primers were synthesized by Thermo sher (Table S1).
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