1260 in nity

Biomass used for chemotaxonomic studies except fatty acid was obtained from cultures grown on ISP2 plate containing 10% (w/v) NaCl for 14 days at 37 °C . The diagnostic isomers of diaminopimelic acid were determined by HPLC according to methods used by Tang et al. (2009) and using TLC to verify it (Hasegawa et al.1983 , Lechevalier et al.1970 ). The whole-cell sugar pattern and peptidoglycan amino acids were detected by HPLC according to methods used by Tang et al. (2009) . The respiratory quinones were isolated using the method of Collins et al. (1977) and analysed by HPLC (Agilent Technologies 1260 In nity) (Groth et al.1996) . Polar lipids were extracted and then separated by using two dimensional TLC and identi ed using previously described procedures (Minnikin et al.1984; Hasegawa et al.1983 ). Molybdophosphoric acid, molybdenum blue, ninhydrin and α-naphthol were used for the detection of total polar lipids, phospholipids, aminolipids and glycolipids, respectively. For cellular fatty acid analysis, strain YIM 93776 T was grown on tryptic soya agar (TSA; Difco) with 10% (w/v) NaCl at 37°C and harvested after 7 days. Fatty acid methyl esters were extracted, methylated and analysed by using the Microbial Identi cation System (Sherlock version 6.1; MIDI database: TSBA6) according to the manufacturer's instructions (Sasser et al.1990 ).

Amino-acids contents were ascertained by High Performance Liquid Chromatography (HPLC-DAD at λ = 338 nm; λ = 208 nm, Agilent 1260 In nity), according to ISO 3903/d ISO 17180-2013 (MO/09 V02).
2.2.3. Crude fat (Folch method , MO/02 v01)
The lipid content was determined gravimetrically following an extraction of crude fat from 1 g of each sample according to the way characterized elsewhere (Folch et al. 1957; Khemir et al. 2020 ) using chloroform: methanol (2:1, v/v) solution containing 0.01% butylhydroxytoluene (BHT).
2.2.4. Fatty acids (ISO 12966-4 2015 and ISO 12966-2 2017, MO/03) To ascertained the fatty acid content, fatty acid methyl esters were recovered from the removed fat according to the standard ISO 12966-2:2017 procedure that comprises dissolution of glycerides in isooctane and trans-esteri cation via potassium hydroxide methanol solution, and then resolved using gas chromatography in the guise of the ISO 12966-2 (version 2012) and ISO 12966-4 (version 2015) (MO/03).

The production of L-homoserine and ectoine were quanti ed by high-performance liquid chromatography (HPLC, Agilent Technologies 1260 In nity, U.S.A.). After fermentation, the culture broth samples were centrifuged to separate the supernatant from the fermentation cultures at 12,000 g for 10 min and then ltered using a 0.22 μm aqueous membrane. The supernatant containing product and byproduct was diluted appropriately for subsequent analysis. Precolumn O-phthalaldehy (OPA) derivatization was used to determine the concentration of L-homoserine as described previously [23] . Before the HPLC detection, total 800 μL OPA derivatization reagent was mixed with the broth supernatant for one minute. HPLC detection was conducted at a column temperature of 40 • C and the detection wavelength of 338 nm with a Phenomenex Luna C18 SB-Aq column (ODS 5 μm, 20 × 150 mm, Agilent, U.S.A.). 50 mM potassium dihydrogen phosphate/acetonitrile (81:19, v/v) was used as the mobile phase for L-homoserine detection at a ow rate of 1.0 mL/min, and the detection time was 15 min. The OD value of each sample was determined at 600 nm in triplicate using a Hitachi U-1100 spectrophotometer.
The concentration of glucose in the broth was determined at 505 nm using an enzyme-coupled glucose assay kit (Rsbio, China). All values were average of three independent experiments.