Stably Cas9-expressing HepG2 cell line (catalog no.: T3256), lentiviral sgRNA targeting human LDLR (catalog no.: 264181110204), and nonviral plasmids containing sgRNA targeting individual candidate genes were purchased from ABM Goods, Inc (Canada). Whole-genome lentiviral pooled CRISPR-Cas9 KO library (catalog no.: 73178-LV) was obtained from Addgene (Cambridge, MA). Alexa Fluor™ 568 carboxylic acid succinyl ester, Alexa Fluor™ 488-Human Transferrin conjugate (catalog no.: T13342), and DiI-human LDL conjugate (catalog no.: L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-quantitative PCR (qPCR) were purchased from Applied Biosystems (Foster City, CA). Monensin, sodium salt (catalog no.: M5273), was purchased from Sigma-Aldrich (St. Louis, MO). Anti-LDLR antibody (catalog no.: 3839) was purchased from BioVision (Milpitas, CA), anti-TAGLN antibody (catalog no.: GT336) from Invitrogen (Carlsbad, CA), and anti-PCSK7 antibody (catalog no.: 19346S) from Cell Signaling Technology (Danvers, MA).
Alexa fluor 488 human transferrin conjugate
Manufactured by Thermo Fisher Scientific
Alexa Fluor™ 488-Human Transferrin conjugate is a fluorescent labeling reagent used to label and track human transferrin protein. It consists of the Alexa Fluor™ 488 dye covalently attached to human transferrin. The conjugate can be used to monitor transferrin localization and uptake in cell-based assays.
Automatically generated - may contain errors
Lab products found in correlation
Monensin sodium salt, by Merck Group (2 mentions)
Dii human ldl conjugate, by Thermo Fisher Scientific (2 mentions)
Anti tagln antibody, by Thermo Fisher Scientific (2 mentions)
Anti pcsk7 antibody, by Cell Signaling Technology (2 mentions)
Anti ldlr antibody, by Abcam (2 mentions)
Alexa fluor tm 568 carboxylic acid succinyl ester, by Thermo Fisher Scientific (1 mentions)
2 protocols using alexa fluor 488 human transferrin conjugate
1
High-throughput LDLR Regulation Screening
2
CRISPR/Cas9-Mediated Functional Screening
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Stably Cas9-expressing HepG2 cell line (Cat# T3256), lentiviral sgRNA targeting human LDLR (Cat# 264181110204) and non-viral plasmids containing sgRNA targeting individual candidate genes were purchased from ABM Goods, Inc. (Canada). Wholegenome lentiviral pooled CRISPR/Cas9 knockout library (Cat# 73178-LV) was obtained from Addgene (Cambridge, MA). Alexa Fluor TM 568 carboxylic acid succinyl ester, Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI-human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City, CA). Monensin, sodium salt, (Cat# M5273) was purchased from Sigma-Aldrich (St. Louis, MO). Anti-LDLR antibody (Cat# 3839) was purchased from BioVision (Milpitas, CA), anti-TAGLN antibody (Cat# GT336) from Invitrogen (Carlsbad, CA), and anti-PCSK7 antibody (Cat# 19346S) from Cell Signaling Technology (Danvers, MA).
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