Kim 1 human elisa kit

The urinary concentrations of TIMP-2 and IGFBP7 were measured using the NephroCheck TM test (Astute Medical, San Diego, CA, USA) as previously described. 21 Urinary NGAL concentrations were measured by ARCHITECT Urine NGAL assay (Abbott Diagnostics, Abbott Park, IL, USA) using an ARCHITECT i2000. KIM-1 concentrations were measured by KIM-1 (human) ELISA kit (Enzo Life Sciences GmbH, Lörrach, Germany) and calprotectin concentrations by PhiCal ® Calprotectin ELISA kit (Immundiagnostik AG, Bensheim, Germany). 16, 22 Statistical analysis Due to non-normal distribution and small sample size, numeric data are presented as median and interquartile range. Nonparametric Mann-Whitney U test was performed for comparison of two groups. For comparison of more than two groups, nonparametric Kruskal-Wallis test with post-hoc Dunn's test was applied. Comparison of categorical parameters was conducted by Pearson's χ 2 test or, in case of less than five patients per group, by Fisher's exact test. Receiver operating characteristics (ROC) curves were calculated to evaluate the diagnostic accuracy of the investigated biomarkers. P < 0.05 was regarded as statistically significant. All statistical analyses were performed using IBM ® SPSS ® Statistics Version 22 and SAS ® Version 9.4 WIN (Cary, NC, USA).

Plasmatic heart fatty acid binding protein (h-FABP) was detected with a rat h-FABP ELISA (both from Hycult Biotech), while aspartate aminotransferase (AST) and creatinine were detected with IFCC colorimetric assay and the kinetic Jaffé method, respectively (both from Roche Diagnostics) in the central lab of the UZ Leuven. In human samples, urinary kidney injuy molecule-1 (KIM-1) and neutrophil-gelatinase associated lipocalin (NGAL) were measured by ELISA per the manufacturer"s instructions (KIM-1 (human) ELISA kit, dilution 1/500, detection limit 1.279 pg/ml, Enzo Life Sciences, Lause, Switzerlandand -NGAL (human) ELISA KIT; dilution 1/500, detection limit 4 pg/mL; Bioporto, Gentofte, Denmark). Plasma h-FABP was measured by ELISA per the manufacturer"s instructions (dilution 1/10; ELISA HK402, detection limit 102 pg/mL, Hycult Biotech, Uden, the Netherlands).