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D α tocopherol polyethylene 1000 succinate

Manufactured by Merck Group

D-α-Tocopherol polyethylene 1000 succinate is a synthetic derivative of vitamin E. It is a pale yellow, viscous liquid that is used as a laboratory reagent and in various industrial applications. The core function of this compound is to serve as an antioxidant and emulsifier.

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2 protocols using d α tocopherol polyethylene 1000 succinate

1

Xenograft Model of Rhabdomyosarcoma

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Athymic nude mice (Crl; Nu-Foxn1Nu/Nu) were implanted with 2 million TC32 cells in the left gastrocnemius muscle in a volume of 100 μL Hank’s Balanced Salt Solution (Thermo-Fischer Scientific). Once tumor diameter reached 0.5 cm, mice were randomly assigned to either the control, mithramycin, PHA-767491, or combination groups. Mithramycin was administered via an Alzet 1003D osmotic pump (Durect) placed within the peritoneum which delivered a dose continuous dose in a vehicle of DPBS with MgCl2 and CaCl2 (MilliporeSigma) and was removed after treatment. PHA-767491 was administered via oral gavage in 200 μL of its vehicle, 1 mg/10 mL D-α-Tocopherol polyethylene 1000 succinate (MilliporeSigma). Mice were euthanized by CO2 asphyxiation when tumor diameter reached greater than 2 cm3. Tumor size was determined by the equation( Dd26*3.12 ), where D is the maximum diameter and d is the minimum diameter. All animal work was approved by the Van Andel Institute Animal Care and Use Committee.
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2

Preparation and Storage of Mithramycin and PHA-767491

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Mithramycin (Tocris Bioscience) was dissolved as a 1 mg/mL stock solution in PBS, and frozen in aliquots. PHA-767491 and other small molecule inhibitors (Selleck Chemicals) were dissolved in DMSO as stock solutions. Small molecule stocks were stored at −80 °C for storage or −20 °C for experimental use, minimizing freeze-thaw cycles. For in vivo experiments, a 30 mg/mL stock solution of PHA-767491 (Adooq Bioscience) was made by dissolving the powder in a solution of 1 mg/10mL D-α-Tocopherol polyethylene 1000 succinate (MilliporeSigma) for oral gavage.
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