A Phenom ProX scanning electron microscope (Phenom-World B.V., The Netherlands) with the Element Identification (EID) software package was used to characterize PEEK samples at microscale and to perform the Energy Dispersive Spectrometer (EDS). Before starting with SEM observation, a Desk Sputter Coater (Phenom-World B.V., The Netherlands) has been used to sputter the PEEK samples with gold (150 A).
Desk sputter coater
Manufactured by Thermo Fisher Scientific
The Desk Sputter Coater is a versatile laboratory instrument used for the deposition of thin conductive layers onto a variety of substrates. It operates by sputtering, a physical vapor deposition process that creates a uniform coating on the target surface.
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Lab products found in correlation
3 protocols using desk sputter coater
1
Microscale Characterization of PEEK Samples
2
Sputter Coating for SEM Imaging
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Before starting with SEM observation, a Desk Sputter Coater (Phenom-World B.V., Eindhoven, The Netherlands) was used to sputter the samples with gold (150 A).
A Phenom ProX scanning electron microscope (Phenom-World B.V., The Netherlands) was used to characterize all samples at microscale and to visualize the biofilm formation on the discs.
A Phenom ProX scanning electron microscope (Phenom-World B.V., The Netherlands) was used to characterize all samples at microscale and to visualize the biofilm formation on the discs.
3
Preparation of LCWB Samples for SEM
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The treated and untreated LCWB samples were fixed with glutaraldehyde, dehydrated with ascending concentrations of ethanol, then immersed in hexamethyldisilazane (HMDS, Sigma-Aldrich, Milan, Italy) for 10 min, twice. HMDS was decanted from the specimen vial and the tissues were left to air dry at room temperature. The dried samples were subjected to gold-sputtering with a desk sputter coater (Phenom-World B.V., The Netherlands) and then observed with SEM (Phenom-World B.V., The Netherlands) at different magnifications, as previously described [23 (link),24 (link)].
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