Morning fasted blood samples were obtained from the antecubital vein for the analyses of hemoglobin, ferritin, and serum total 25-hydroxyvitamin D, e.g., serum 25(OH)D. Blood for the hemoglobin analysis was drawn into EDTA tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) and immediately further analyzed with Sysmex XP300 analyzer (SysmexCo., Kobe, Japan). For serum ferritin, the blood was drawn into Vacuette gel serum tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) and centrifuged for 10 min with 3,600 rpm to collect serum, which was then frozen to −20°C for further analysis. The samples were analyzed with Siemens Immulite 2000 XPI analyzer (Siemens Healthcare Lianberis, United Kingdom), where the serum ferritin was determined by using immunometric chemiluminescence method. The sensitivity of the assay for ferritin was 0.4 µ/L and the precision (CV%) for the assay was 4.6%. The measurements of serum 25(OH)D were performed using electrochemiluminescence immunoassays (ECLIA).
Xp 300 analyzer
Manufactured by Sysmex
Sourced in Japan, United States
The XP-300 analyzer is a compact hematology analyzer designed for small to medium-sized laboratories. It performs complete blood count (CBC) analysis, including red blood cells, white blood cells, and platelets. The XP-300 provides accurate and reliable results with a small sample size.
Automatically generated - may contain errors
7 protocols using xp 300 analyzer
1
Fasted Blood Analysis for Hemoglobin, Ferritin, and Vitamin D
2
Fasting Blood Biomarkers Analysis
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Fasting blood samples were obtained from an antecubital vein for analysis of hemoglobin, cortisol, leptin, triiodothyronine (T3), insulin, insulin-like growth factor 1 (IGF-1), and glucose. PRE and POST samples from each subject were collected at the same time of day between 7am and 9am. Blood was drawn into EDTA tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) for analyses of hemoglobin that were completed immediately with Sysmex XP300 analyzer (SysmexCo., Kobe, Japan). For the determination of serum hormone concentrations, blood was drawn into Vacuette gel serum tubes (Greiner-Bio-One GmbH, Kremsmünster, Austria) and centrifuged at 3600 rpm for 10 min to collect serum, which was frozen at -20ºC. Concentrations of cortisol, leptin, T3, insulin, and IGF-1 were analyzed by an immunometric chemiluminescense method (Immulite 2000 XPi, Siemens Healthcare, United Kingdom). The assay sensitivities were 5.5 nmol • L -1 (cortisol), 8.2 ug • L -1 (leptin), 1.5 pmol • L -1 (T3), 2 U • L -1 (insulin), 1.7 nmol • L -1 (IGF-1), and 2.0 mmol
3
Platelet Protein Binding Assay
4
Automated Blood Count Analysis
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Complete blood count and platelets analysis were carried out using automated Sysmex XP-300 analyzer with strict adherence to the manufacturer instruction. Daily quality control was carried out to validate each test run as described by equipment manufacturer [33 ].
5
Platelet Binding Assay Protocol
6
Accurate Malaria Parasite Density Estimation
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In brief, malaria parasites were screened, using malaria rapid diagnostic test (RDT) kits as described by the manufacturers (Standard Diagnostics Bioline, 2013). Regardless of RDT results, the blood smear microscopy method was used for confirmation. Blood sample collection, malaria diagnosis, and hematological parameters determination procedures had been previously described [27 (link)]. Malaria parasite density was determined more accurately using the actual white blood cells count per microliter, unlike the assumed white blood cells count of 8000 WBC/μl estimation recommended by the WHO [28 ]:
Hematological parameters and platelets were estimated using an automated Sysmex XP-300 analyzer. To produce quality results with precision and accuracy, daily analysis of EIGHTCHECK control stabilized blood consisting of low, medium, and high levels of controls was done according to manufacturer instruction [29 ].
Hematological parameters and platelets were estimated using an automated Sysmex XP-300 analyzer. To produce quality results with precision and accuracy, daily analysis of EIGHTCHECK control stabilized blood consisting of low, medium, and high levels of controls was done according to manufacturer instruction [29 ].
7
Comprehensive Blood Biomarker Assessment
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Venous blood was collected from cardiac puncture into well-labelled EDTA and gel separator tubes for hematological (full blood count) assessments by using Sysmex XP-300 analyzer and biochemical (liver, kidney, and alpha-fetoprotein test) assessments using Mindray BS-230 auto analyzer. Also, prothrombin time was measured by using a Wondfo Finecare analyzer.
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