7600 210 automatic biochemical analyzer

Venous blood or follicular fluid samples were centrifuged at 3,000 × g for 10 min to isolate the upper layer for analysis. Serum sex hormones, including follicle-stimulating hormone, luteinizing hormone, estradiol, progesterone and testosterone, were analyzed using commercially available kits from the Unicel DXI 800 Access immunoassay system (Beckman Coulter). Total cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein levels were determined using commercially available kits purchased from Randox Laboratories Ltd., Northern Ireland, United Kingdom. The assays were carried out on a Hitachi 7600–210 Automatic Biochemical Analyzer (Hitachi Corporation, Japan). Commercially available kits for free fatty acid (FFA) determination were purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China).

The serum level of hsCRP was measured by an immunoturbidimetry method using Cobas (Roche, Germany). In the workshop by Centers for Disease Control and American Heart Association in 2002, the cut-points of hsCRP concentrations for purposes of cardiovascular risk assessment were classified as follows; low risk (<1.0 mg/L), average risk (1.0 to 3.0 mg/L), and high risk (>3.0 mg/L) [6 (link)]. In the Jupiter trial, participants who were indicated by hsCRP levels >2 mg/L categorized to high vascular risk [28 (link)]. High hsCRP was defined as hsCRP levels > 2.0 mg/L based on the previous studies [6 (link),28 (link)]. In an epidemiological follow-up data from National Health and Nutrition Examination Survey, deaths due to ischemic heart disease in women significantly increased when SUA levels were in the highest quartile (5.6 mg/dL) compared with the lowest quartile (4.0 mg/dL) [17 (link)]. The Princeton school district family study defined the participants who had hyperuricemia when they had above a 90th percentile of SUA level [29 (link)]. The level of SUA was measured by Uricase, a colorimetry method using a Hitachi 7600-210 automatic biochemical analyzer (Hitachi, Japan). High SUA level was defined as SUA levels exceeding the upper 90th percentile (=5.6 mg/dL) based on the previous studies [17 (link),29 (link)].