Povidone k 90

Two batches of GMO, batch number G1017 (lot numbers ZG0467 and WA1499) were purchased from Spectrum Chemicals; pure GMO was purchased from Sigma-Aldrich. Poloxamer 407 was purchased from Spectrum Chemicals, whereas low molecular weight chitosan and citric acid were purchased from Sigma-Aldrich. Povidone K 90 (polyvinyl pyrrolidone) was purchased from BASF Chemicals. Polyethylene glycols (PEG), Eudragit E100, Eudragit L100, Eudragit S100, hydroxypropyl methyl cellulose (HPMC), mineral oil, and olive oil purchased from Fisher Scientific. Deionized water was used for all the experiments. Physical mixtures used for comparative analysis were prepared by geometrically mixing the lipids/excipients in the same amount as utilized in the preparation of solid lipid nanoparticles.

VGH was obtained as a free sample from Wockhardt Research Centre, Aurangabad (M.S), India, and lactose monohydrate was obtained from DFE Pharma, India. Povidone K-90 was procured from BASF, Ahmadabad, India, Colloidal silicon dioxide from Evonik, Mumbai, India Caprylocaproyl polyoxyl-8 glycerides from Gattefosse, Mumbai, India Magnesium stearate from Mallinckrodt New Delhi, India, Cellulose Acetate from Eastman Chemicals Ltd., Singapore, Hydroxypropyl Cellulose from Nippon Soda, Tokyo, Japan and polyethylene glycol from Clariant Chemicals India Limited, Vadodara, India. Isopropyl alcohol and acetone were purchased from Lee chang, Bengaluru, India and Merck, Bengaluru, India respectively.