Log In Sign up
The largest database of trusted experimental protocols

Cstf 77

Manufactured by Fortis Life Sciences
Visit Supplier

The CSTF-77 is a lab equipment product designed for precision cell separation and tissue fractionation. It utilizes centrifugal separation technology to isolate specific cell types or tissue components from complex biological samples. The core function of the CSTF-77 is to provide efficient and reliable sample preparation for downstream analysis and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

Cfim 68, by Fortis Life Sciences (2 mentions) Cpeb1, by Cell Signaling Technology (2 mentions) Cstf 64, by Fortis Life Sciences (2 mentions) β actin, by Merck Group (2 mentions) Bca assessment, by Thermo Fisher Scientific (2 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Phototope horseradish peroxidase western blot detection kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using cstf 77

1

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates were prepared from −80°C-stored cell
pellets with RIPA lysis buffer, on ice. Following brief sonication, lysates were
clarified by 12,000 × g centrifugation for 10–15 min. Samples
were loaded according to total protein content, determined by BCA assessment
(Thermo Pierce), and within each cell type the amounts were normalized to the
first mock-infected sample (corresponding to ~100,000 cells total per
lane). Antibodies and dilutions used: CSTF-64 (A301-092A), CSTF-77 (A301-096A),
and CFIm-68 (A301-356A): Bethyl Laboratories, 1:2000. CPEB1: Cell
Signaling Technologies (13583), 1:1000. β-actin: Sigma-Aldrich (clone
AC-15, A1978), 1:10,000. Ms anti IE (CH160): Virusys, 1:5000. Ms anti UL57:
Virusys, 1:1000. Ms anti UL99(pp28): Virusys, 1:1000. Ms anti UL83(pp65)
Virusys, 1:5000.
Batra R., Stark T.J., Clark A.E., Belzile J.P., Wheeler E.C., Yee B.A., Huang H., Gelboin-Burkhart C., Huelga S.C., Aigner S., Roberts B.T., Bos T.J., Sathe S., Donohue J.P., Rigo F., Ares M J.r., Spector D.H, & Yeo G.W. (2016). RNA binding protein CPEB1 remodels host and viral RNA landscapes. Nature structural & molecular biology, 23(12), 1101-1110.
+ Open protocol
+ Expand
2

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates were prepared from −80°C-stored cell
pellets with RIPA lysis buffer, on ice. Following brief sonication, lysates were
clarified by 12,000 × g centrifugation for 10–15 min. Samples
were loaded according to total protein content, determined by BCA assessment
(Thermo Pierce), and within each cell type the amounts were normalized to the
first mock-infected sample (corresponding to ~100,000 cells total per
lane). Antibodies and dilutions used: CSTF-64 (A301-092A), CSTF-77 (A301-096A),
and CFIm-68 (A301-356A): Bethyl Laboratories, 1:2000. CPEB1: Cell
Signaling Technologies (13583), 1:1000. β-actin: Sigma-Aldrich (clone
AC-15, A1978), 1:10,000. Ms anti IE (CH160): Virusys, 1:5000. Ms anti UL57:
Virusys, 1:1000. Ms anti UL99(pp28): Virusys, 1:1000. Ms anti UL83(pp65)
Virusys, 1:5000.
Batra R., Stark T.J., Clark A.E., Belzile J.P., Wheeler E.C., Yee B.A., Huang H., Gelboin-Burkhart C., Huelga S.C., Aigner S., Roberts B.T., Bos T.J., Sathe S., Donohue J.P., Rigo F., Ares M J.r., Spector D.H, & Yeo G.W. (2016). RNA binding protein CPEB1 remodels host and viral RNA landscapes. Nature structural & molecular biology, 23(12), 1101-1110.
+ Open protocol
+ Expand
3

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extracts from cells or tissue samples were subjected to SDS-PAGE and transferred to polyvinylidene difluoride membrane (Millipore). Antibody against BID (Cat# 2002, RRID: AB_10692485) was from Cell Signaling Technology. Antibody against ZFP36L2 (Cat# sc-365908, RRID: AB_10847357) was from Santa Cruz Biotechnology. Antibody against CstF64 (Cat# A301-092A, RRID: AB_873014), CstF77 (Cat# A301-096A, RRID: AB_873019), and CPSF100 (Cat# A301-582A, RRID: AB_1078863) was from Bethyl Laboratories. Membranes were incubated overnight at 4 C with primary antibody and visualized with a phototope-horseradish peroxidase Western Blot Detection kit (Thermo Fisher Scientific).
Lin A., Ji P., Niu X., Zhao X., Chen Y., Liu W., Liu Y., Fan W., Sun Y., Miao C., Zhang S., Tan W., Lin D., Wagner E.J, & Wu C. (2021). CstF64-Induced Shortening of the BID 3'UTR Promotes Esophageal Squamous Cell Carcinoma Progression by Disrupting ceRNA Cross-talk with ZFP36L2. Cancer research, 81(22).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!