Pro-inflammatory cytokines TNF-α and IL-6 were quantified by using the specific mouse ELISA kits (BD Biosciences, Franklin Lakes, NJ, USA) in plasma and lung tissue. Plasma levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were measured using commercial assay kits (Pointe Scientific, Lincoln Park, MI, USA) according to the manufacturer’s instructions.
Specific mouse elisa kits
Manufactured by BD
Sourced in United States
Specific mouse ELISA kits are laboratory equipment used to detect and quantify specific proteins or analytes in mouse samples. These kits provide a standardized and reliable method for measuring the levels of target molecules in biological samples. The core function is to facilitate the measurement of target analytes through the principles of enzyme-linked immunosorbent assay (ELISA) technology.
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Lab products found in correlation
4 protocols using specific mouse elisa kits
1
Cytokine and Enzyme Quantification
2
Quantifying Inflammatory Markers and Liver Enzymes
3
Cytokine and Liver Enzyme Profiles
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Serum levels of alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatinine, and blood urea nitrogen (BUN) were determined using colorimetric assays (Pointe Scientific, Canton, MI). Serum and tissue levels of interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) were determined using mouse-specific ELISA kits (BD Biosciences, San Diego, CA). All assays were carried out according to manufacturer’s instructions. Cytokine measurements have larger sample sizes because cytokine levels were also determined in a small replication cohort.
4
Serum Biomarker Quantification Protocol
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Whole-blood samples were centrifuged at 4,000 g for 10 min to collect serum. The activity of aspartate aminotransferase (AST) was determined using a commercial assay kit (Pointe Scientific, Lincoln Park, MI). Serum and tissue levels of interleukin 1β (IL-1β) and interleukin 6 (IL-6) were determined using mouse-specific ELISA kits (BD Biosciences, San Diego, CA). All assays were carried out according to manufacturer’s instructions.
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