Tcrβ apc e780 h57 597

Manufactured by Thermo Fisher Scientific

The TCRβ APC-e780 (H57-597) is a flow cytometry antibody that binds to the beta chain of the T cell receptor (TCR) complex. It is used for the identification and characterization of T cells in various research applications.

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Lab products found in correlation

Live dead aqua, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg af488, by Thermo Fisher Scientific (1 mentions) Cd8α pacific blue, by BioLegend (1 mentions) Eomes pe, by Thermo Fisher Scientific (1 mentions) Pe texas red, by Thermo Fisher Scientific (1 mentions) Ifn γ percp cy5, by BioLegend (1 mentions) Cd62l apc mel 14, by BioLegend (1 mentions) Brdu flow kit, by BD (1 mentions) H57 597, by BioLegend (1 mentions) C myc y69, by Abcam (1 mentions) M1 69, by BD (1 mentions) Cd24 percpcy5, by Thermo Fisher Scientific (1 mentions) Ki67 pe cy7, by BD (1 mentions) T bet pecy7, by Thermo Fisher Scientific (1 mentions) Cd45.2 pacific blue, by BioLegend (1 mentions) Rorγt bv421, by BD (1 mentions)

Spelling variants of this product

TCRβ (H57-597) (20 citations) H57-597 (10 citations) TCRβ APC (6 citations)

2 protocols using tcrβ apc e780 h57 597

Multiparameter Flow Cytometry Analysis

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The following antibodies were used (from BD Biosciences unless otherwise noted): CD8α-Pacific Blue or AlexaFluor (AF)700 (53-6.7, Biolegend); CD4 fluoroscein isothiocyanate (FITC) (GK1.5, eBiosciences), allophycocyanin (APC) (RM4-5), phycoerythrin (PE)-Cy5 or PE-Cy7 (RM4-5, Biolegend), or PE-TexasRed (RM4-5, Invitrogen), TCRβ APC-e780 (H57-597, eBiosciences), IL4Ra PE (mIL4R-M1), CD62L APC (MEL-14),, CD44 AF700 (IM7, Biolegend), CD122-biotin (TM-1) followed by streptavidin-PE Texas Red, Eomes-PE or AF647 (Dan11mag, eBiosciences), IFNγ-PerCPCy5.5 (XMG1.2, Biolegend), APC or PE, pSTAT6 PE (J1-773.58.11), and pAkt T308 PE (J1-223.371). For pS6 staining, cells were stained with a rabbit anti-phospoS6 monoclonal (2F9; Cell Signaling) and then stained with AF488 goat anti-rabbit IgG (Invitrogen). Live/Dead Aqua (Invitrogen) was used to exclude dead cells.

Carty S.A., Koretzky G.A, & Jordan M.S. (2014). Interleukin-4 Regulates Eomesodermin in CD8+ T Cell Development and Differentiation. PLoS ONE, 9(9), e106659.

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Comprehensive Immune Cell Phenotyping

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The following antibodies were used: APC labeled PBS57 loaded CD1d-tetramers from the NIH tetramer facility, TCRβ APC-e780 (H57-597, eBiosciences) or Pacific Blue (H57-597, Biolegend), CD44 AlexaFluor(AF)700 (IM7, Biolegend), CD45.2 Pacific Blue (104, Biolegend), Thy1.1 phycoerythrin (PE) (A85-1, BD Pharmingen), Thy1.2 FITC (53-2.1, BD Pharmingen), NK1.1 PE-Cy7 or PE (PK136, BD Pharmingen), or Pacific Blue (PK136, Biolegend), or NK1.1-biotin (PK136, eBiosciences) followed by streptavidin-PE Texas Red, CD24 PerCPCy5.5 (M1/69, eBiosciences) or FITC or PE (M1/69, BD Pharmingen), IL-17A PE (17B7, eBiosciences), IL-4 PE-Cy7 (BVD6-24G2, eBiosciences), IFN-γ PerCPCy5.5 (XMG1.2, Biolegend), cMyc (Y69, abcam) or p27^kip1 (D69C12; Cell Signaling Technology) followed by anti-rabbit IgG PE (Life Sciences), Ki67-PeCy7 (B56, BD Pharmingen), rabbit IgG isotype (Life Technologies) and BrdU (BrdU flow kit; BD Pharmingen). For transcription factor staining, cells were incubated with antibody to PLZF (D-9, Santa Cruz), followed by anti-mouse IgG1 PE (A85-1, eBiosciences), and then T-bet PE-Cy7 (4B10, eBiosciences), GATA3 PerCpCy5.5 (TWAJ, eBiosciences), and RORγt-BV421 (Q31-378, BD Biosciences).

Sklarz T., Guan P., Gohil M., Cotton R.M., Ge M.Q., Haczku A., Das R, & Jordan M.S. (2017). mTORC2 regulates multiple aspects of NKT-cell development and function. European journal of immunology, 47(3), 516-526.

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