Acetyl ester cm h2dcfda

T2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH), fluorescein disodium salt, Folin-Ciocalteu reagent, Gallic acid, catechin, 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA), ethanol, acetone, isopropanol, hydrochloric acid (HCl) were purchase from Sigma-Aldrich. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), antimycin A, Dulbecco Modified Eagle Medium (DMEM) and Phosphate buffer saline (PBS) were purchase from Invitrogen (Waltham, MA, USA).

Antibody against BCO2 (Catalog number [Cat#] 14324-1-AP, 1:1000) was acquired from ProteinTech Group (Rosemont, IL, USA). Antibodies against cleaved caspase-3 (Cat# 9662, 1:1000), p53 (Cat# 2524, 1:1000), Bax (Cat#2772, 1:1000) and Bcl-2 (Cat#15071, 1:1000) were obtained from Cell Signaling (Danvers, MA, USA). Antibody to β-actin (Cat# A5441, 1:10,000) was obtained from Sigma Aldrich (St Louis, MO, USA). The 5-(and-6)-chloromethyl-2′, 7′-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H₂DCF-DA) and N-acetyl cystenine (NAC) were purchased from Sigma Aldrich. Lutein was obtained from Kemin Health (Des Moines, IA, USA; FloraGLO Lutein 10% VG TabGrade). Beta-carotene and astaxanthin were obtained from Sigma-Aldrich. Working solutions of carotenoids were freshly prepared with dimethyl sulfoxide (DMSO) and tetrahydrofurlan (THF) in a ratio 2:1 immediately before use. Docetaxel and paclitaxel were obtained from Tocris Bioscience (Minneapolis, MN, USA).