Liver, jejunum and adipose tissues were embedded in FSC 22 frozen section compound (Leica Biosystems, Richmond, IL, USA), then frozen and sectioned at 5 mm using a Leica CM1860 Cryostat (Leica Microsystems, Nussloch, Germany). Sections were then stained with oil red O solution or hematoxylin and eosin (Cayman chemical, USA), after which they were mounted on silicone-coated slides (Leica, USA) and examined using an Olympus BX61 microscope (Tokyo, Japan) and photographed using an Olympus DP70 digital camera (Tokyo, Japan).
Fsc 22 frozen section compound
Manufactured by Leica
Sourced in Germany, United States
The FSC 22 frozen section compound is a specialized laboratory product designed for cryogenic sectioning. It is formulated to provide a stable and consistent support medium for delicate tissue samples during the freezing process, enabling thin, uniform sections to be obtained for microscopic analysis.
Automatically generated - may contain errors
Lab products found in correlation
Dp70 digital camera, by Olympus (4 mentions)
Bx61 microscope, by Olympus (3 mentions)
Silicone coated slides, by Leica (2 mentions)
Cm1860 cryostat, by Leica (1 mentions)
Cryomicrotome, by Leica (1 mentions)
P mlc2, by Cell Signaling Technology (1 mentions)
Fasudil, by Selleck Chemicals (1 mentions)
Tissue tek cryomold, by Sakura Finetek (1 mentions)
P erk1 2 p44 p42, by Cell Signaling Technology (1 mentions)
Indium tin oxide, by Bruker (1 mentions)
2 5 dihydroxybenzoic acid dhb, by Bruker (1 mentions)
Cm1850 cryostat, by Leica (1 mentions)
Oil red o solution, by Cayman Chemical (1 mentions)
Streptavidin alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
Fv10i microscope, by Olympus (1 mentions)
Vectastain abc standard kit, by Vector Laboratories (1 mentions)
Ra3 6b2, by Thermo Fisher Scientific (1 mentions)
Immpact dab, by Vector Laboratories (1 mentions)
Oct compound, by Sakura Finetek (1 mentions)
Hematoxylin and eosin, by Muto Pure Chemicals (1 mentions)
10 protocols using fsc 22 frozen section compound
1
Histological Analysis of Tissue Samples
2
Histological Analysis of Liver and Aorta
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 8 weeks of treatments, the liver tissues and aorta were collected, fixed in 10% neutral buffered (formaldehyde), and sliced (5 μm thick) and trimmed transversely into serial sections using a cryo-microtome (Leica Biosystems, Richmond, VA, USA). Sections were later deparaffinized in xylene, rehydrated through an ascending ethanol series, and stained with hematoxylin-eosin (H&E). Oil Red-O staining was used to analyze fat deposition in liver tissues. The liver tissues were embedded in FSC 22 frozen section compound (Leica Biosystems, Richmond, VA, USA) and sectioned (5 μm thickness) using a cryostat (Tissue-Tek® Cryo3® Flex Microtome/Cryostat, Sakura, Leiden, The Netherlands). The sections were stained with Oil Red-O staining, and mounting was performed with xylene-based media. The slides were observed under an Olympus BX 61 microscope (Olympus, Tokyo, Japan) at 200× magnification and images were acquired using an Olympus DP70 digital camera (Olympus).
3
Proteomic Analysis of ROCK2 Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetonitrile (ACN), trifluoroacetic acid (TFA) and general chemicals were from Merck (Darmstadt, Germany) or Sigma-Aldrich (Steinheim, Germany). Fasudil was from Selleckchem (Houston, TX). Antibodies were phosphorylated (P) P-ROCK2 (T249, #ab83514, Abcam, Cambridge, UK), Ki67 (#550609), unphosphorylated/general (G) G-ROCK2 (#610624) (both from BD Biosciences, Heidelberg, Germany), RHOA (STA-403-A-CB, Biocat, Heidelberg, Germany), P-MLC2 (#3671), P-ERK1/2(p44/p42) (#4370), G-ERK1/2(p44/p42) (#9102), P-P38 (#4511), G-P38 (#9218) (all from Cell Signaling), HSP90 (sc-7947, Santa Cruz Biot., CA). MALDI peptide calibration standard II (#222570), 2,5-dihydroxybenzoic acid (DHB, #209813) and indium tin oxide (ITO) slides were from Bruker Daltonik (Bremen, Germany), Isopentane (GPR RECTAPUR) from VWR (Darmstadt, Germany), FSC22 Frozen Section Compound from Leica Biosystems (Wetzlar, Germany) and Tissue-Tek Cryomolds from Sakura Finetek (Heppenheim, Germany). [18F]-FDG was purchased from ZAG Zyklotron AG (Karlsruhe, Germany).
4
Histochemical Analysis of Skin Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For histochemical analysis, the skin models were submerged in 22 × 22 mm Peel-A-Way disposable plastic tissue embedding molds (Science Services, Munich, Germany) containing FSC 22 frozen section compound (Leica Biosystems, Wetzlar, Germany) and flash-frozen in liquid nitrogen. Models were stored at least one night at −80°C before further processing. Samples were then cut into 7-μm sections using a Cryostat CM1850 (Leica Biosystems, Wetzlar, Germany) at −20°C against the direction of application (from dermis to epidermis) and put onto glass slides for subsequent histological staining. At least 30 sections were prepared from each skin model and analyzed for each histochemical staining.
5
Frozen Liver Tissue Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen liver tissues embedded in FSC 22 frozen section compound (Leica, USA) were sectioned at 8 mm, mounted on silicone coated slides (Leica, USA), and then stained with oil red O solution (Cayman chemical, USA) for 10 min at 60°C and Mayer’s hematoxylin solution for 1 min. All of the slides were observed under an Olympus BX61 microscope (Tokyo, Japan) and photographed using an Olympus DP70 digital camera (Tokyo, Japan).
6
Brain Tissue Preparation for ISH and IHC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were deeply anesthetized with sodium pentobarbital (50 mg/kg, i.p.) and then perfused transcardially with 4% (w/v) paraformaldehyde (PFA) in phosphate-buffered saline (PBS). The brains were removed and post-fixed in the same fixative and stored at 4 °C overnight, followed by cryoprotection of 30% (w/v) sucrose in PBS for 2 days, embedded in FSC22 Frozen Section Compound (Leica, Wetzlar, Germany) and stored at – 80 °C until cryosectioning. Brains were cryosectioned coronally at a thickness of 40 µm. Every third section from the serial sections was processed for ISH combined with IHC.
7
Immunofluorescence Staining of Intestinal Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For Fig. 1E , small intestines were embedded in FSC22 frozen section compound (Leica Microsystems). Five μm cryosections were labelled with rat anti-human Ep-CAM (G8.8; BioLegend) Ab and biotinylated armenian hamster anti-TCRβ (H57-597; BioLegend) Ab, and then labelling was visualised with streptavidin-Alexa Fluor 594 (Invitrogen) and Alexa Fluor 647-conjugated anti-rat IgG (H+L) Ab (Invitrogen). For Fig. 6B , small intestines from Xcr1+/venus mice were first fixed with 4% paraformaldehyde and then embedded in FSC22 frozen section compound. Five μm cryosections were labelled with rat anti-mouse CD4 Ab (BioLegend) and labelling visualised with goat anti-Rat IgG Ab conjugated-Alexa Fluor 594 (Invitrogen). Sections were examined using the FV10i microscope (Olympus).
8
Histological Analysis of Skin Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cross-sections of the full-thickness skin specimens were collected on the last day of the experiment. Skin samples were fixed for 24 h in 10% (v/v) formaldehyde and embedded in FSC 22 Frozen Section Compound (Leica Microsystems, Wetzlar, Germany). Vertical sections of 5 μm thickness were fixed onto glass slides and stained with hematoxylin and eosin (H & E) and the Masson’s trichrome stain to observe morphology and collagen formation, respectively. The slides were examined by light microscopy (Olympus BX53; Olympus Corp., Tokyo, Japan), and the images were digitally captured at a 1360 × 1024 pixel resolution with an Olympus DP70 digital camera (Olympus Corp., Tokyo, Japan).
9
Immunohistochemical Analysis of Lymphoid Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thymus and spleen were fixed with 4% paraformaldehyde and embedded in OCT compound (Sakura Finetek) or FSC22 frozen section compound (Leica Microsystems), and 5 μm cryosections were prepared. The sections were stained with hematoxylin and eosin (Muto Pure Chemicals). To detect B and T cells, sections were stained with biotinylated Abs against B220 (RA3-6B2, eBioscience 13-0452-85, 1 ug mL−1) or Thy1.2 (30-H12, BD Bioscience 553011, 1 ug mL−1) after blockade of endogenous peroxidase with 3% H2O2/methanol. Biotinylated Abs were developed with VECTASTAIN ABC Standard kit and ImmPACT DAB (Vector Laboratories). Hematoxylin was used as counterstain.
10
Quantifying Superoxide Anion in Rat Eyes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The eyes were dissected from the rats 24 h after LPS injection and frozen fresh in FSC22 frozen section compound (Leica Microsystems, Wetzlar, Germany) with liquid nitrogen. Dihydroethidium (DHE, Sigma), an oxidative fluorescent dye, was used for immunohistochemical detection of cytosolic superoxide anion (O2−). ROS production was determined by conversion of DHE to ethidium bromide. Square areas of ICB microphotographs (1.44 megapixels in size) were selected, and mean gray values of the DHE signal were evaluated with ImageJ software, as we reported recently [21 (link)]. Two whole ICB areas were evaluated in each eye and then four eyes were examined in each group.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!