Liver, jejunum and adipose tissues were embedded in FSC 22 frozen section compound (Leica Biosystems, Richmond, IL, USA), then frozen and sectioned at 5 mm using a Leica CM1860 Cryostat (Leica Microsystems, Nussloch, Germany). Sections were then stained with oil red O solution or hematoxylin and eosin (Cayman chemical, USA), after which they were mounted on silicone-coated slides (Leica, USA) and examined using an Olympus BX61 microscope (Tokyo, Japan) and photographed using an Olympus DP70 digital camera (Tokyo, Japan).
Fsc 22 frozen section compound
Manufactured by Leica Biosystems
Sourced in United States, Germany
The FSC 22 frozen section compound is a product designed for use in cryogenic sectioning applications. It functions as a mounting medium to adhere and support tissue samples during the frozen sectioning process. The compound is formulated to provide a stable, supportive base for thin tissue sections to be cut on a cryostat or other freezing microtome.
Automatically generated - may contain errors
Lab products found in correlation
Bx61 microscope, by Olympus (2 mentions)
Dp70 digital camera, by Olympus (2 mentions)
Leica cm1860 cryostat, by Leica Microsystems (1 mentions)
Silicone coated slides, by Leica camera (1 mentions)
Cryomicrotome, by Leica Biosystems (1 mentions)
P mlc2, by Cell Signaling Technology (1 mentions)
Fasudil, by Selleck Chemicals (1 mentions)
Tissue tek cryomold, by Sakura Finetek (1 mentions)
P erk1 2 p44 p42, by Cell Signaling Technology (1 mentions)
Indium tin oxide, by Bruker (1 mentions)
2 5 dihydroxybenzoic acid dhb, by Bruker (1 mentions)
Cm1850 cryostat, by Leica Biosystems (1 mentions)
4 protocols using fsc 22 frozen section compound
1
Histological Analysis of Tissue Samples
2
Histological Analysis of Liver and Aorta
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After 8 weeks of treatments, the liver tissues and aorta were collected, fixed in 10% neutral buffered (formaldehyde), and sliced (5 μm thick) and trimmed transversely into serial sections using a cryo-microtome (Leica Biosystems, Richmond, VA, USA). Sections were later deparaffinized in xylene, rehydrated through an ascending ethanol series, and stained with hematoxylin-eosin (H&E). Oil Red-O staining was used to analyze fat deposition in liver tissues. The liver tissues were embedded in FSC 22 frozen section compound (Leica Biosystems, Richmond, VA, USA) and sectioned (5 μm thickness) using a cryostat (Tissue-Tek® Cryo3® Flex Microtome/Cryostat, Sakura, Leiden, The Netherlands). The sections were stained with Oil Red-O staining, and mounting was performed with xylene-based media. The slides were observed under an Olympus BX 61 microscope (Olympus, Tokyo, Japan) at 200× magnification and images were acquired using an Olympus DP70 digital camera (Olympus).
3
Proteomic Analysis of ROCK2 Signaling
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Acetonitrile (ACN), trifluoroacetic acid (TFA) and general chemicals were from Merck (Darmstadt, Germany) or Sigma-Aldrich (Steinheim, Germany). Fasudil was from Selleckchem (Houston, TX). Antibodies were phosphorylated (P) P-ROCK2 (T249, #ab83514, Abcam, Cambridge, UK), Ki67 (#550609), unphosphorylated/general (G) G-ROCK2 (#610624) (both from BD Biosciences, Heidelberg, Germany), RHOA (STA-403-A-CB, Biocat, Heidelberg, Germany), P-MLC2 (#3671), P-ERK1/2(p44/p42) (#4370), G-ERK1/2(p44/p42) (#9102), P-P38 (#4511), G-P38 (#9218) (all from Cell Signaling), HSP90 (sc-7947, Santa Cruz Biot., CA). MALDI peptide calibration standard II (#222570), 2,5-dihydroxybenzoic acid (DHB, #209813) and indium tin oxide (ITO) slides were from Bruker Daltonik (Bremen, Germany), Isopentane (GPR RECTAPUR) from VWR (Darmstadt, Germany), FSC22 Frozen Section Compound from Leica Biosystems (Wetzlar, Germany) and Tissue-Tek Cryomolds from Sakura Finetek (Heppenheim, Germany). [18F]-FDG was purchased from ZAG Zyklotron AG (Karlsruhe, Germany).
4
Histochemical Analysis of Skin Models
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For histochemical analysis, the skin models were submerged in 22 × 22 mm Peel-A-Way disposable plastic tissue embedding molds (Science Services, Munich, Germany) containing FSC 22 frozen section compound (Leica Biosystems, Wetzlar, Germany) and flash-frozen in liquid nitrogen. Models were stored at least one night at −80°C before further processing. Samples were then cut into 7-μm sections using a Cryostat CM1850 (Leica Biosystems, Wetzlar, Germany) at −20°C against the direction of application (from dermis to epidermis) and put onto glass slides for subsequent histological staining. At least 30 sections were prepared from each skin model and analyzed for each histochemical staining.
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