Mouse melanoma cells (B16F10), mouse breast cancer cells (4T1) and human cancer cell lines (MCF-7, MDA-MB-231, A875, A375, SK-MEL-28, SKBR-3, A2058) were purchased from ATCC. All cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin/streptomycin in a humidified atmosphere of 5% CO2 at 37 °C. Mycoplasma testing was routinely performed every 3 months by using Mycoplasma PCR Detection Kit (Beyotime, C0301S).
Female C57BL/6 mice (20 ± 2 g), female Balb/c mice (18 ± 2 g) and nude mice (18 ± 2 g) were purchased from the Laboratory Animal Center of the Third Military Medical University. All animal experiments were performed according to the rules of the Laboratory Animal Welfare and Ethics Committee of the Third Military Medical University (assigned number: AMUWEC2020477). Mice were housed with a 12 h light/dark cycle with 40–60% humidity at 20–24 °C, and with free access to food and water. The humane endpoint was established as body weight loss exceeding 20% and tumor size greater than 2 cm. Mice were sacrificed by intraperitoneal injection of an overdose of sodium pentobarbital (200 mg/kg) to relieve pain.
Female C57BL/6 mice (20 ± 2 g), female Balb/c mice (18 ± 2 g) and nude mice (18 ± 2 g) were purchased from the Laboratory Animal Center of the Third Military Medical University. All animal experiments were performed according to the rules of the Laboratory Animal Welfare and Ethics Committee of the Third Military Medical University (assigned number: AMUWEC2020477). Mice were housed with a 12 h light/dark cycle with 40–60% humidity at 20–24 °C, and with free access to food and water. The humane endpoint was established as body weight loss exceeding 20% and tumor size greater than 2 cm. Mice were sacrificed by intraperitoneal injection of an overdose of sodium pentobarbital (200 mg/kg) to relieve pain.