Log In Sign up
The largest database of trusted experimental protocols

Global dna methylation 5 mc elisa easy kit

Manufactured by Epigentek
Visit Supplier
Sourced in United States

The Global DNA Methylation (5-mC) ELISA Easy kit is a laboratory tool designed to quantify the global level of 5-methylcytosine (5-mC) in genomic DNA samples. It utilizes an enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the amount of 5-mC present in the DNA.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Thermo Fisher Scientific (1 mentions) Nanodrop one, by Thermo Fisher Scientific (1 mentions) Fitamp general tissue section dna isolation kit, by Epigentek (1 mentions)

Spelling variants of this product

MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (44 citations) MethylFlash Global DNA Methylation (5-methylcytosine [5-mC]) ELISA Easy Kit (4 citations) MethyFlash Global DNA Methylation (5-mC) ELISA Easy kit (2 citations)

2 protocols using global dna methylation 5 mc elisa easy kit

1

Global DNA Methylation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole genomic DNA was extracted from primary astrocytes (2 × 106) using the Blood and Cultured Cell DNA Extraction Kit (EpiGentek, P-1018, Farmingdale, NY, USA) according to the manufacturer’s protocol. The purity of the extracted DNA was determined by measuring the absorbance with a Nanodrop spectrophotometer (Thermo Scientific, Waltham, MA, USA). Further analysis of global DNA methylation was performed using a Global DNA Methylation (5-mC) ELISA Easy kit (EpiGentek, P-1030, Farmingdale, NY, USA). In this experiment, methylated fractions of DNA from the treatment groups were analyzed by using detection antibodies and quantified by measuring the absorbance with a microplate reader (Thermo Scientific, Waltham, MA, USA). The amount of methylated DNA was proportional to the measured OD intensity.
Sivalingam K, & Samikkannu T. (2020). Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes. Brain Sciences, 10(9), 611.
+ Open protocol
+ Expand
2

Embryonic Cardiac DNA Methylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Global DNA methylation as measured as a percentage of 5-methylcytosine (5-mC %) was assayed in the DNA extracted from hearts from each cohort of 3 day and 8 day embryos. The cohorts were embryos treated in ovo with (1) ethanol, (2) the vehicle saline, (3) GSH alone, and (4) ethanol plus GSH. To obtain adequate DNA from hearts of day 3 (HH stage 19–20) embryos, we pooled 5–6 hearts per sample and analyzed 8 samples per cohort. For the 8 day hearts, one heart was used per sample. DNA was extracted using the FitAmp General Tissue Section DNA Isolation Kit (Epigentek), DNA concentration was determined by NanodropOne (ThermoFisher), and the global DNA methylation assay [Epigentek, Global DNA Methylation (5-mC) ELISA Easy Kit] was used. Samples were measured in duplicate and the average taken to quantify the percentage DNA methylation. The standard curve was generated from the standard methylated DNA provided with the assay kit over the amount of total DNA.
Jawaid S., Strainic J.P., Kim J., Ford M.R., Thrane L., Karunamuni G.H., Sheehan M.M., Chowdhury A., Gillespie C.A., Rollins A.M., Jenkins M.W., Watanabe M, & Ford S.M. (2021). Glutathione Protects the Developing Heart from Defects and Global DNA Hypomethylation Induced by Prenatal Alcohol Exposure. Alcoholism, clinical and experimental research, 45(1), 69-78.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!