Hpv16 e6

Manufactured by GeneTex

HPV16-E6 is a protein that is part of the human papillomavirus (HPV) type 16. It plays a crucial role in the viral life cycle and is involved in the immortalization and transformation of human cells.

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Lab products found in correlation

Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Polyvinylidene difluoride membrane, by Cytiva (1 mentions) Hybond nitrocellulose membrane, by Cytiva (1 mentions) Gapdh, by SCBT (1 mentions) Hpv16 e7, by Santa Cruz Biotechnology (1 mentions) Hpv18 e6, by Santa Cruz Biotechnology (1 mentions) Hpv18 e7, by Abcam (1 mentions) Species specific hrp conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions)

2 protocols using hpv16 e6

Western Blot Analysis of Cellular Proteins

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Protein extracts from randomly selected samples were quantified using Coomassie (Beyotime, Cat. no: ST1119) and 20 μg of total protein was used for western blots. Proteins were separated on 10% SDS-PAGE gels (BioSci, Cat. no: 8012011) and transferred onto polyvinylidene difluoride membranes (Cytiva Life Sciences, Cat. no: 10600023). Membranes were blocked in 5% BSA for 1 h and incubated overnight at 4°C with antibodies specific for: RB (1:500, Abclonal, Cat. no: A11409), p53 (1:500, Abclonal, Cat. no: A16989), HPV16-E6 (1:1,000, Genetex, Cat. no: 132686), HPV16-E7 (1:1,000, Genetex, Cat. no: 133411;), GAPDH (1:1,000, Abclonal, Cat. no: A19056), followed by HRP-conjugated secondary antibodies (1:5,000, Abclonal, Cat. no: AS014 and Cat. no: AS003) for 1 h at room temperature. Bands were visualized using a Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific; Cat. no: 32209).

Fan J., Fu Y., Peng W., Li X., Shen Y., Guo E., Lu F., Zhou S., Liu S., Yang B., Qin X., Hu D., Xiao R., Li X., Yang S., Yuan C., Shu Y., Huang H., Wan T., Pi Y., Wang S., Chen W., Wang H., Zhong L., Yuan L., Wen B., Kong B., Mills G.B., Zou D., Xia B., Song K., Chen G., Ma D, & Sun C. (2023). Multi-omics characterization of silent and productive HPV integration in cervical cancer. Cell Genomics, 3(1), 100211.

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Western Blot Analysis of Protein Expression

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Equal amounts of protein were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto Hybond nitrocellulose membrane (Amersham biosciences), followed by immunoblot analysis. Antibodies used in this study were as follows: CREB1 (9197, CST), phospho‐CREB1 (9198, CST), HPV16 E6 (GTX132686, GeneTex), HPV16 E7 (sc‐65711, Santa Cruz), HPV18 E6 (sc‐365089, Santa Cruz), HPV18 E7 (ab100953, Abcam), Snail (3879, CST), Slug (9585, CST), GAPDH (G‐9, SCBT), GFP (sc‐9996, SCBT), and FLAG (F3165, Sigma). Western blots were visualized with species‐specific HRP conjugated secondary antibodies (Jackson ImmunoResearch) and ECL (Thermo/Pierce).

Li Y., Patterson M.R., Morgan E.L., Wasson C.W., Ryder E.L., Barba‐Moreno D., Scarth J.A., Wang M, & Macdonald A. (2023). CREB1 activation promotes human papillomavirus oncogene expression and cervical cancer cell transformation. Journal of Medical Virology, 95(8), e29025.

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