Immunohistochemical (IHC) analysis was performed using standard protocols on an automated Ventana Benchmark XT immunostaining system (Roche‐Ventana, Darmstadt, Germany). We used primary antibodies against glial fibrillary acidic protein (GFAP; rabbit polyclonal, Agilent/Dako, Glostrup, Denmark), microtubule‐associated protein 2 (Map2; mouse monoclonal (HM‐2), Sigma‐Aldrich, St. Louis, MO, USA), p53 protein (mouse monoclonal (DO‐7), Agilent/Dako, Glostrup, Denmark), Olig‐2 (goat polyclonal, R&D Systems, Abingdon, UK), epithelial membrane antigen (mouse monoclonal (E‐29), Agilent/Dako, Glostrup, Denmark), p65 RelA (rabbit monoclonal (D14E12), Cell signaling, Danvers, U.S.A.), L1CAM (mouse monoclonal, (UJ127.11), Sigma–Aldrich, St Louis, MO, USA), Claudin‐1 (mouse monoclonal (ab56417), Abcam, Cambridge, UK), Ki67 (mouse monoclonal (Ki‐67P), Dianova, Hamburg, Germany), phospho‐histone‐3 (rabbit polyclonal, Bioclare Medical, Hague, Netherlands) and NF (mouse monoclonal (2F11), Agilent/Dako).
Ki 67p
Manufactured by Dianova
Sourced in Germany
Ki‐67P is a laboratory equipment product that measures the Ki‐67 protein expression. Ki‐67 is a marker associated with cellular proliferation. The Ki‐67P product allows for the quantification of Ki‐67 protein levels.
Automatically generated - may contain errors
Lab products found in correlation
Ab56417, by Abcam (1 mentions)
Rela p65, by Cell Signaling Technology (1 mentions)
L1cam, by Merck Group (1 mentions)
Ventana benchmark xt, by Roche (1 mentions)
Olig2, by R&D Systems (1 mentions)
Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions)
Egfr pharmdx kit, by Agilent Technologies (1 mentions)
K5005, by Agilent Technologies (1 mentions)
2 protocols using ki 67p
1
Comprehensive Immunohistochemical Profiling
2
Histopathological Analysis of Tumor Samples
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The animals were sacrificed by cervical dislocation. The tumors were collected along with the adjoining skin. In case that no tumor was macroscopically seen, the skin from the site of tumor cell injection was prepared. In addition, the spleen, liver, lung, heart, intestine, and kidneys were also collected. After formol fixation, the organs and the tumor samples were embedded in paraffin for histological examination. The samples were cut into 4‐μm slices and were stained with hematoxylin–eosin (HE) and immunohistologically for the determination of the EGFR expression level (EGFR pharmDx™ Kit; DAKO, Hamburg, Germany). Ki‐67 proliferation index (monoclonal antibody Ki‐67P; Dianova, Hamburg, Germany) was also determined for the tumor samples and detected with secondary reagents (K5005; DAKO). The tissues were examined for any histopathological alterations, which might be due to toxin‐induced injury using the criteria as published elsewhere (von Mallinckrodt et al., 2014 ).
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