Anti fhit antibody

The human cDNAs of various Gα subunits were obtained from Guthrie Research Institute (Sayre, PA). Wild-type Fhit in pCMV-SPORT6 was purchased from Invitrogen (Carlsbad, CA). Gα_16/z chimeras were constructed by overlapping PCR which swapped the corresponding regions of Gα₁₆ with Gα_z as described previously [15 (link)]. Cell culture and Lipofectamine PLUS reagents, and anti-Fhit antibody were purchased from Invitrogen (Carlsbad, CA). Anti-Gα₁₆ was obtained from Gramsch Laboratories (Schwabhausen, Germany). Anti-Gα_q/11 antibody was purchased from Calbiochem (San Diego, CA). Anti-α-tubulin, anti-HA, and anti-Flag antibodies as well as anti-HA affinity gel were from Sigma-Aldrich (St. Louis, MO). Antisera against PLCβ1/2/3 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Other antibodies were purchased from Cell Signaling Technology (Danvers, MA). Protein G-agarose was from Thermo Fisher Scientific (Rockford, IL). ECL kit was from Amersham Biosciences (Piscataway, NJ).

The anti-Fhit antibody was purchased from Invitrogen (Waltham, MA, USA). Antibodies against bcl-2, cleaved caspase-3, cleaved caspase-8, cleaved PARP, beclin-1, LC3B, and DYKDDDDK (FLAG) tag were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against bcl-xL, pan 14-3-3 (K-19), 14-3-3γ (KC21), 14-3-3σ (5D7), 14-3-3τ (C-17), and 14-3-3ζ (C-16) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-p62 antibody was obtained from Abnova (Taipei, Taiwan). Anti-β-actin and anti-FLAG M2 antibodies were purchased from Sigma (St. Louis, MO, USA).