Primary antibodies used in this study included mouse anti-TH (Millipore, Temecula, CA, MAB318), rabbit anti-GAPDH (Cell Signaling, Danvers, MA, 2118), rabbit anti-4-HNE (Alpha diagnostics, San Antonio, TX, HNE11-S), rabbit anti-HO-1 (Millipore, Temecula, CA, AB1284), rabbit anti-pJNK (Cell Signaling, Danvers, MA, 9251), rabbit anti-JNK (Cell Signaling, Danvers, MA, 9252). Secondary antibodies used in this study included anti-mouse/rabbit HRP-linked secondary antibody (Cell Signaling, Danvers, MA, 7076 or 7074), Alexa Fluor 488 donkey anti-mouse secondary antibody (Life technologies, Eugene, OR, A21202), Alexa Fluor 568 donkey anti-rabbit secondary antibody (Life technologies, Eugene, OR, A10042), goat anti-mouse or goat anti-rabbit (Millipore, Temecula, CA, AP124 or AP132), and mouse or rabbit PAP antibody (Jackson Immunoresearch, West Grove, PA). PQ dichloride hydrate (Sigma, St. Louis, MO, 856177) was purchased and dissolved in saline for mice treatment.
Pq dichloride hydrate
Manufactured by Merck Group
PQ dichloride hydrate is a chemical compound used as a laboratory reagent. It is composed of pyridine and chlorine, and exists as a hydrated crystal. The compound is typically used in various chemical and biochemical applications that require its specific properties and characteristics.
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Lab products found in correlation
Rabbit anti jnk, by Cell Signaling Technology (1 mentions)
Rabbit anti p jnk, by Cell Signaling Technology (1 mentions)
Anti mouse rabbit hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
Alexa fluor 568 donkey anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Rabbit anti 4 hne, by Alpha Diagnostic (1 mentions)
Alexa fluor 488 donkey anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions)
Mouse anti th, by Merck Group (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Primerscript rt reagent kit perfect real time kit, by Takara Bio (1 mentions)
Bulge loop mirnaqrt pcr primer kit, by RiboBio (1 mentions)
Sybr premix ex taq 2 kit, by Takara Bio (1 mentions)
2 protocols using pq dichloride hydrate
1
Protein Expression and Oxidative Stress
2
Nrf2 and microRNA-380-3p Regulation in MPTP-Induced Neurodegeneration
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PQ dichloride hydrate (99.2% w/w) and MPTP (99.99% w/w) were purchased from Sigma-Aldrich Co. (St. Louis, MO). The terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) kit and MaxvisionTM (HRP-polymer anti-rabbit IHC kit) were purchased from Fuzhou Maixin Biotech. Co. Ltd. (China). Anti-Nrf2 (C-20, sc-722) and anti-TH (sc-374047) antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). A Bulge-LoopTM miRNA QRT–PCR Primer kit was purchased from RiboBio Co. Ltd. (Guangzhou, China). A Primer Script RT Reagent Kit (Perfect Real-Time) kit and a SYBR Premix Ex Taq II kit were purchased from TaKaRa Biotechnology Co. Ltd. (Japan). A MicroRNA ISH Buffer, Controls Kit, and a LNA™ microRNA-380-3p probe were purchased from Exiqon Life Sciences, Life Sciences and Diagnostics Co. (Denmark). QRT–PCR primers for microRNA-380-3p were purchased from Guangzhou RiboBio Co., Ltd (China). Goat anti-Rabbit-DIG-AP IgG and NBT/BCIP staining solutions were purchased from Roche Co. (USA). Sheep serum was purchased from Millipore Corporation (Temecula, CA).
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