Plasma membrane proteins were extracted from mouse kidneys as previously described [10 (link)], and were size-fractionated by SDS/PAGE (40μg/lane) and transferred to nitrocellulose membrane. Western blot analyses were performed using anti-renin) antibody (MyBioSourc #315812). Appropriate horseradish peroxidase-conjugated IgGs (Thermo Scientific, Rockford, IL) were used as secondary antibodies. The bands were visualized by chemiluminescence method (Invitrogen, Carlsbad, CA) and captured on light-sensitive imaging film (Denville Scientific Inc, Metuchen, NJ).
Appropriate horseradish peroxidase conjugated iggs
Manufactured by Thermo Fisher Scientific
Appropriate horseradish peroxidase-conjugated IgGs are laboratory reagents that consist of immunoglobulin G (IgG) molecules chemically conjugated to the enzyme horseradish peroxidase. These conjugated IgGs are commonly used as detection and labeling agents in various immunoassay techniques.
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Lab products found in correlation
2 protocols using appropriate horseradish peroxidase conjugated iggs
1
Extraction and Analysis of Kidney Plasma Membrane Proteins
2
Western Blot Analysis of KBAT Protein
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KBAT-specific antibodies (Xu et al., 2011 (link); Rahmati et al., 2013 (link)) were used for Western blot analysis. Briefly, microsomal membrane proteins were prepared from cerebellum of WT and KBAT KO mice. Thereafter, proteins were size-fractionated by SDS/PAGE (50 µg/lane) and transferred to nitrocellulose membrane. Western blot analyses were performed using anti-KBAT (Slc26a11) antibodies. Appropriate horseradish peroxidase-conjugated IgGs (Thermo Scientific) were used as secondary antibodies. The bands were visualized by chemiluminescence method (Invitrogen), and captured on light-sensitive imaging film (Denville Scientific).
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