Annexin 5 conjugated alexa fluor 488 apoptosis detection kit

Annexin V is a protein that binds to phosphatidylserine (PS) residues which are exposed on cell surfaces of apoptotic, but not normal cells. During apoptosis, the PS groups are exposed to the exterior of the cell membrane. This binding of PS with Annexin V is an established biochemical marker of apoptosis. Induction of apoptosis caused by yeast and paclitaxel in EAC-primary tumors was quantitatively determined through flow cytometry using the Annexin V-conjugated Alexa Fluor 488 Apoptosis Detection kit following the manufacturers instructions (BD Biosciences).

Annexin-V is a protein that binds to phosphatidylserine (ps) residues that are exposed on cell surfaces of apoptotic, but not normal, cells. During apoptosis, the ps groups are exposed to the exterior of the cell membrane. This binding of ps with Annexin-V is an established biochemical marker of apoptosis. Induction of apoptosis caused by Biobran treatment in gastric cancer cells was quantitatively determined through flow cytometry using the Annexin-V-conjugated Alexafluor 488 Apoptosis Detection Kit following the manufacturer’s instructions (BD Biosciences, San Jose, CA). The early apoptotic cells stained with Alexa488 give green fluorescence and present in the lower right quadrant of the fluorescence-activated cell-sorting histogram, and the late apoptotic cells stained with both Alexa488 and propidium iodide give red-green fluorescence and present in the upper right quadrant of the fluorescence-activated cell-sorting histogram.