Polyclonal anti fsp1

Monoclonal antibodies against Snail-1, ERK1/2, phospho-ERK1/2, phospho-SMAD1-5-8, and Cav1 were from Cell Signaling Technology; monoclonal antibodies against E-cadherin, β-catenin, and γ-catenin were from BD (Becton-Dickinson Laboratories, Mountain View, CA); monoclonal antibodies against occludin was from Invitrogen (Carlsbad, CA); monoclonal antibodies against tubulin, α-SMA, α-catenin, vimentin, pan-cytokeratin, and fibronectin were from Sigma (Saint Louis, MO); monoclonal anti-N-cadherin and polyclonal anti-ZO-1 were from Zymed (Invitrogen, Carlsbad, CA); polyclonal anti-actin, -SMAD2-3, and -phospho-MEK were from Santa Cruz Biotechnology (CA); and polyclonal anti-phospho SMAD2-3 was from Biosource (Camarillo, CA). Monoclonal anti-CD54 was from Biolegend (San Diego, CA); polyclonal anti-Fsp1 was from Dako (Glostrup, Denmark); monoclonal anti-CD31 from Serotec (Oxford, UK); and polyclonal anti-Ki67 from Abcam (Cambridge, UK). Monoclonal anti-JAM-A clone BV-11 was a gift from Dr. E. Dejana (Milan Italy). Fluor 647?phalloidin and Hoechst 33342 were from Invitrogen (Carlsbad, CA). CI 1040 was from Selleck (Houston, TX).

Monoclonal antibodies against SNAI-1 (#3895), YAP (#14074) phospho-SMAD2/3 (#8828), SMAD2/3 (#8685), phospho-SMAD3 (#9520) and CAV1 (#3267) were from Cell Signaling Technology; monoclonal antibodies against α-SMA (#A2547) and pan-cytokeratin (#SRE0061) were from Sigma Aldrich (Saint Louis, MO); monoclonal anti GAPDH (#MAB374) was from Millipore (Burlington MA, USA); monoclonal anti-TEF-1 (sc-376113), polyclonal anti-RhoGDI (sc-360) and anti-CTGF (sc-14939) were from Santa Cruz Biotechnology; polyclonal anti-ZO-1 (#617-300) was from Zymed (Invitrogen, Carlsbad, CA); polyclonal anti-FSP1 (#A5114) was from Dako (Glostrup, Denmark); monoclonal anti-calretinin (#ab92341) was from Abcam; anti-MMP2 (#IM33) was from Merck (Darmstadt, Germany); monoclonal anti-human podoplanin (#566456) and FITC Mouse Anti-Human CD45 Clone HI30 (#55548) were from BD Bioscences (Franklin Lakes, NJ); secondary antibodies Alexa Fluor 488, 555, 574 and 594 (Thermofisher Scientific, Massachusetts, USA); Hoechst 33342 and 4,6-diamidino-2-phenylindole (DAPI) were from Invitrogen (Carlsbad, CA). SB431542 was from Selleck (Houston, TX). 1D11.16 anti-TGFβ1 mAb (BE0057) was from inVivoMab/Bio X Cell (Lebanon, NH).