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Cobas 8000 c702 chemistry analyzer

Manufactured by Roche
Sourced in Switzerland

The Cobas 8000 c702 Chemistry Analyzer is a fully automated clinical chemistry analyzer designed for high-volume testing in clinical laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests, providing accurate and reliable results. The analyzer is equipped with advanced technology to ensure efficient and streamlined workflow, enabling laboratories to meet their testing demands.

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5 protocols using cobas 8000 c702 chemistry analyzer

1

Comprehensive Metabolic Profiling in Body Composition Monitoring

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The results of laboratory investigations (blood and urine) performed on the day that patients underwent body composition monitoring were collected from their electronic medical records. The hemoglobin (Hb) concentration in whole blood samples was determined using an ADVIA 2120i hematology system (Siemens Healthcare Diagnostics, Erlangen, Germany). The levels of total cholesterol, total protein, serum albumin, uric acid, blood urea nitrogen, sCr, sodium, potassium, calcium, phosphorus, and total CO2 in the serum samples were measured on a Cobas 8000 c702 Chemistry Analyzer (Roche Diagnostics, Rotkreuz, Switzerland). The urine protein to Cr ratio was calculated using random urine samples. Cystatin C was measured using an automated AU-5800 analyzer (Beckman Coulter, Brea, CA, USA). The estimated GFR (eGFR) was calculated using the CKD-Epidemiology Collaboration (CKD-EPI) formula [26 (link)].
Diabetes was defined as a fasting blood glucose level of ≥126 mg/dL, glycated Hb of ≥6.5%, or the use of an antidiabetic agent. Hypertension was defined as a systolic or diastolic blood pressure of ≥140 mmHg or ≥90 mmHg, respectively, or the use of antihypertensive agents. Body mass index (BMI, kg/m2) was calculated using the weight and height at the time of body composition measurement and was classified into five groups based on the criteria for the Asian population.
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2

Serum Biomarkers in db/db Mice

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At the end of the 5 weeks treatment period, the db/db mice were fasted overnight and a blood sample was collected by cardiac puncture. After clotting, serum was separated by centrifugation at 4 °C, 3500 rpm for 15 min. Commercial kits were used to measure the serum concentrations of insulin, cholesterol, and TG (Roche, Basel, Switzerland). Absorbance was measured using a Cobas 8000 c702 Chemistry Analyzer (Roche). The serum concentrations of glycated hemoglobin (HbA1c) and FGF21 were determined using commercial kits (Mybiosource, San Diego, CA, USA), according to the manufacturer’s instructions.
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3

Comprehensive Hematological and Biochemical Analyses

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Hematological analysis was determined using a Sysmex XN-9000 analyzer (Sysmex Corporation, Kobe, Japan). Serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, blood urea nitrogen, creatinine and lipid profiles were analyzed using Cobas 8000 C702 chemistry analyzer (Roche, Mannheim, Germany).
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4

Serum Biomarker Analysis in Mice

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After 6 weeks, blood was collected by cardiac puncture immediately after euthanasia and serum was separated by clotting for 30 min at 4 °C and centrifugation at 2000× g for 20 min. The serum concentrations of TG, FFA, cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), and lactate dehydrogenase (LDH) activities were determined using colorimetric assay kits (Roche, Basel, Switzerland). Serum cholesterol, TG, FFA, AST, ALT, and LDH were measured using Cobas 8000 c702 Chemistry Analyzer (Roche, Basel, Switzerland).
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5

Comprehensive Liver and Kidney Panel

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Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine and calcium were measured using the Cobas 8000 C702 chemistry analyzer (Roche, Mannheim, Germany).
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