Cell pellets were lysed using RIPA buffer (Fisher Scientific) supplemented with protease and phosphatase inhibitors (Sigma). 20-30μg protein per sample was run on a polyacrylamide gel and transferred to a PVDF membrane. Proteins were detected using respective primary and HRP-conjugated secondary antibodies, including rabbit anti-IGF1R (Cell Signaling 3027 1:500), rabbit anti-ZWILCH (Abcam 202898 1:1,000), mouse anti-NCL (Abcam 136649 1:1,000), rabbit anti-AUF1 (Cell Signaling 12382 1:1,000), rabbit anti-ELAVL1 (Cell Signaling 12582), rabbit anti-KHSRP (Cell Signaling 5398 1:500), rabbit anti-TIAL1 (Cell Signaling 5137 1:1,000), mouse anti-β-actin (Sigma A5316 1:1,000), goat anti-rabbit (Invitrogen 31460 1:5,000), and goat anti-mouse (Invitrogen 31430 1:5,000).
Rabbit anti elavl1
Manufactured by Cell Signaling Technology
Rabbit anti-ELAVL1 is a primary antibody that recognizes the ELAVL1 (also known as HuR) protein. ELAVL1 is an RNA-binding protein that regulates the stability and translation of target mRNAs. This antibody can be used to detect and study the ELAVL1 protein in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Mouse anti β actin, by Merck Group (2 mentions)
Protease and phosphatase inhibitor, by Merck Group (2 mentions)
Rabbit anti zwilch, by Abcam (2 mentions)
Mouse anti ncl, by Abcam (2 mentions)
Rabbit anti auf1, by Cell Signaling Technology (2 mentions)
Rabbit anti khsrp, by Cell Signaling Technology (2 mentions)
Rabbit anti tial1, by Cell Signaling Technology (2 mentions)
Rabbit anti igf1r, by Cell Signaling Technology (2 mentions)
2 protocols using rabbit anti elavl1
1
Western Blot Protein Detection Protocol
2
Western Blot Protein Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell pellets were lysed using RIPA buffer (Fisher Scientific) supplemented with protease and phosphatase inhibitors (Sigma). 20-30μg protein per sample was run on a polyacrylamide gel and transferred to a PVDF membrane. Proteins were detected using respective primary and HRP-conjugated secondary antibodies, including rabbit anti-IGF1R (Cell Signaling 3027 1:500), rabbit anti-ZWILCH (Abcam 202898 1:1,000), mouse anti-NCL (Abcam 136649 1:1,000), rabbit anti-AUF1 (Cell Signaling 12382 1:1,000), rabbit anti-ELAVL1 (Cell Signaling 12582), rabbit anti-KHSRP (Cell Signaling 5398 1:500), rabbit anti-TIAL1 (Cell Signaling 5137 1:1,000), mouse anti-β-actin (Sigma A5316 1:1,000), goat anti-rabbit (Invitrogen 31460 1:5,000), and goat anti-mouse (Invitrogen 31430 1:5,000).
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